在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Mouse Neurofilament light polypeptide (Nefl)

In Stock
  • 中文名稱:
    小鼠Nefl重組蛋白
  • 貨號:
    CSB-EP015688MO
  • 規格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    65.5 kDa
  • 表達區域:
    2-543aa
  • 氨基酸序列
    SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
  • 基因功能參考文獻:
    1. Study found that NFL protein levels are at least doubled in pmn mutant motoneurons and that NFL depletion rescues defective axon growth in cultured motoneurons and prolongs survival of pmn mutant mice. This effect was found both in Nefl+/-;pmn motoneurons in which elevated NF expression was brought back to wild-type control levels and in Nefl-/-;pmn motoneurons in which axonal neurofilament was completely lost. PMID: 27021905
    2. It may have a role in protecting neurites from dystrophy and in regulating cellular pathways related to the generation of Ab plaques. PMID: 26344875
    3. Nefl(N98S/+) mice had a noticeable tremor, and most animals showed a hindlimb clasping similar to human Charcot-Marie-Tooth Type 2E phenotype. PMID: 25552649
    4. Mice lacking NF-Lr ecapitulated the delayed synapse elimination phenotype observed in micelacking Nfasc155. PMID: 25232125
    5. The finding of this study suggested that a lack of NFL protein alters the expression of cytoskeletal proteins and disrupts other NF subunits, causing intracellular aggregation but not gross structural changes in cortical neurons or cytoarchitecture. PMID: 23172043
    6. Neurofilament light chain (NFL) and neuronal intermediate filament protein alpha-internexin accumulate in axon swellings in the spinal white matter in a superoxide dismutase (SOD)-1 mouse model. PMID: 22609817
    7. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
    8. Data show that mitochondria essentially stopped moving in neurons expressing neurofilament protein (NFL) mutants, probably a consequence of cytoskeletal disruption. PMID: 22155564
    9. NEFL transgenic mice exhibited extended duration of the hindlimb clasping response and gait anomalies, as well as sensorimotor deficits in stationary beam and suspended bar tests PMID: 21168446
    10. Neuropathic effects of overexpressing NF-L can occur at the level of transgene RNA and are mediated by sequences in the NF-L 3' UTR PMID: 12196589
    11. nNOS inhibitor, AR-R17477AR, prevents the loss of NF68 immunoreactivity induced by methamphetamine in the mouse striatum PMID: 12675928
    12. The 3' untranslated region of light neurofilament (NF-L) transcript enhances the reactivity of its own translated product and leads to loss of solubility and aggregation of NF-L protein and to coaggregation of mutant superoxide dismutase 1 (SOD1) protein PMID: 15028764
    13. copper-mediated NF-L modification may be closely related to oxidative reactions which play a critical role in neurodegenerative diseases PMID: 15388232
    14. We observed three overlapping phases in NF-L transgenic mice, including transient aggregate formation, reactive microgliosis, and progressive motor neuron loss. PMID: 15920739
    15. p190RhoGEF is involved in aggregation of NF-L protein and support a working hypothesis that aggregation of p190RhoGEF and NF-L is an upstream event triggering neurotoxicity in motor neuron disease. PMID: 16236762
    16. Mouse lactotrophs, gonadotrophs, thyrotrophs and somatotrophs express NF68 in a sexually dimorphic manner. Mouse pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
    17. Alpha-internexin coassembles with all 3 neurofilament proteins into a single network of filaments in quadruple-transfected cells. PMID: 17005864
    18. NFL gene deficiency could retard MSCs proliferation and neuronal generation, even though the capability of neuronal lineage differentiation of MSCs may not be deterred. PMID: 17395374
    19. Disruption of neurofilament network with aggregation of NFL is a common triggering event of motor neuron degeneration in Charcot-Marie-Tooth disease. PMID: 17881652
    20. Results suggest a preferential participation of P/Q-type Ca(2+) channels and hence alpha 1A subunits, in regulating spontaneous Ca(2+) transients in anterior pituitary cells under conditions where the proportion of NF68-expressing cells is high. PMID: 17927665
    21. Results suggests that acrolein-mediated NF-L aggregation might be closely related to oxidative reactions, thus these reactions may play a critical role in neurodegenerative diseases. PMID: 18823586
    22. LANP directly regulates expression of the neurofilament light chain, an important neuron-specific cytoskeletal gene, by binding to the promoter of this gene and modulating histone acetylation levels PMID: 19136565
    23. These results support that TDP-43 is involved in neurofilament mRNA metabolism and transport, and provide insight into the pathogenesis of motor neuron death in ALS in which NFL mRNA levels are selectively suppressed. PMID: 19619516

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell projection, axon. Cytoplasm, cytoskeleton.
  • 蛋白家族:
    Intermediate filament family
  • 組織特異性:
    Expressed in the sciatic nerve (at protein level).
  • 數據庫鏈接:


主站蜘蛛池模板: av人摸人人人澡人人超碰小说| 亚洲丁香五月天缴情综合| 一本精品中文字幕在线| 欧美又粗又长又爽做受| 18禁无遮挡羞羞污污污污免费 | 国色天香中文字幕在线视频| 么公的好大好硬好深好爽视频| 性xxxxx大片免费视频| 精品国产乱码久久久久久下载| 亚洲熟妇丰满xxxxx| 丰满人妻一区二区三区免费视频| 成人综合网站| 超碰国产天天做天天爽| 亚洲区激情区无码区日韩区| 亚 洲 视 频 高 清 无 码| 亚洲人成色777777精品音频| 好男人www社区视频在线资源 | 图片区 小说区 区 亚洲五月| 无遮无挡爽爽免费毛片| 午夜亚洲www湿好大| 色综合亚洲一区二区小说性色aⅴ| 青青草视频在线观看| 少妇被黑人到高潮喷出白浆| 99视频有精品视频高清| 上海少妇高潮狂叫喷水了| 国产日韩一区二区三免费高清| 亚洲精品av中文字幕在线| 亚拍精品一区二区三区探花| 97av麻豆蜜桃一区二区| 成人精品视频99在线观看免费| 精品国产一区av天美传媒| 久久视频这里只精品99| 欧美精品videosbestsex日本| 精品国产欧美一区二区| 成人亚洲a片v一区二区三区蜜臀| 国产内射xxxxx在线| 欧美精品v国产精品v日韩精品| 强奷人妻日本中文字幕| 国产成人亚洲综合无码18禁h| 亚洲精品伊人久久久大香 | 97精品久久天干天天|