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Recombinant Mouse NADPH--cytochrome P450 reductase (Por), partial

  • 中文名稱:
    小鼠Por重組蛋白
  • 貨號:
    CSB-YP018378MO
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    小鼠Por重組蛋白
  • 貨號:
    CSB-EP018378MO-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    小鼠Por重組蛋白
  • 貨號:
    CSB-BP018378MO
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    小鼠Por重組蛋白
  • 貨號:
    CSB-MP018378MO
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    PorNADPH--cytochrome P450 reductase; CPR; P450R; EC 1.6.2.4
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • 基因功能參考文獻:
    1. Knockout of the cytochrome P450 reductase by CRISPR/Cas9 technology (POR(-/-)) in HEK293 cells overexpressing Nox4 or Nox5 did not interfere with ROS production in intact cells. However, POR(-/-) abolished the signal in NADPH-stimulated assays using membrane fractions from the very same cells. Moreover, membranes of rat smooth muscle cells treated with angiotensin II showed an increased NADPH-dependent signal with lucigen PMID: 27863990
    2. we observed that miR-378 modulates the oscillation amplitudes of Cdkn1a in the control of cell cycle and Por in the regulation of oxidation reduction by forming partnership with different circadian transcription factors PMID: 26898952
    3. Results are the first to show that some forms of opioid-mediated stress-induced analgesia require brain neuronal P450 activity. PMID: 25020125
    4. Immunological and metabolic changes resulting from a genetic deficiency in CPR expression in the intestine in mice. PMID: 24989705
    5. An apparent link exists between the cytochrome p450 reductase and FGF signaling pathways. PMID: 24086598
    6. These data collectively indicate a novel role of the Cpr gene in fear conditioning and memory. PMID: 22626646
    7. this study provides novel insights into the regulation of gap junction function by CYPOR and suggests that Cx43 may play an important role(s) in CYPOR-mediated bone defects seen in patients. PMID: 21726529
    8. inactivation of the hepatic cytochrome P450 system by conditional deletion of this enzyme PMID: 12566435
    9. Changes in hepatic mRNA expression using microarray analysis and real-time PCR in POR null mice are reported. PMID: 15717863
    10. POR function is indispensable for the proper regulation of retinoic acid levels and tissue distribution not only during early embryonic development but also in later morphogenesis and molecular patterning of the brain, abdominal/caudal region and limbs. PMID: 17126317
    11. The hepatic deficiency of this enzyme reverberates throughout the biological system and produces a coordinated response to the low levels of circulating cholesterol and bile. PMID: 17722906
    12. Data suggest that cellular cytochrome P450 oxidoreductase-dependent cholesterol synthesis is essential during limb and skeletal development. PMID: 19273610

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  • 亞細胞定位:
    Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
  • 蛋白家族:
    NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family
  • 數據庫鏈接:


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