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  4. Recombinant Mouse Krueppel-like factor 8 (Klf8)

Recombinant Mouse Krueppel-like factor 8 (Klf8)

  • 中文名稱：
    小鼠Klf8重組蛋白
  • 貨號：
    CSB-YP803899MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Klf8重組蛋白
  • 貨號：
    CSB-EP803899MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Klf8重組蛋白
  • 貨號：
    CSB-EP803899MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Klf8重組蛋白
  • 貨號：
    CSB-BP803899MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Klf8重組蛋白
  • 貨號：
    CSB-MP803899MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Klf8
  • Uniprot No.：
  • 別名：
    Klf8Krueppel-like factor 8
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-355
  • 氨基酸序列
    MDKFIDNMDV RIKSESGSMQ VFKQVTGPVP TRDPSARADR RNMTSPSFLA ASPMENPALF NDIKIEPPEE LLESDFNMPQ VEPVDLSFHK PKAPLQPASM LQAPIRPPKP PTAPQAIMVP TSADTVTSAA IPTVLTPGSI LASSQGTGGQ PILHVIHTIP SVSLPNKMSG LKTIPLVVQS LPMVYTSLPT DGSPAAITVP LIGGDGKSAG SVKVDPASMC PLEFPSDSDE SAIESGSSAL QSLQGFHHEP ATMVHMQGEE SLDLKRRRIH QCDFAGCSKV YTKSSHLKAH RRIHTGEKPY KCTWDGCSWK FARSDELTRH FRKHTGIKPF RCTDCNRSFS RSDHLSLHRR RHDTM
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
  • 基因功能參考文獻：
    1. Klf8 is highly expressed in various regions of the mouse brain and in particular in the neurons, where it was localized in the cell nuclei. PMID: 26071188
    2. KLF3 and KLF8 have overlapping roles in vivo and participate in the silencing of embryonic globin expression during development. PMID: 23716600
    3. KLF8 is a key component of the transcription factor network that controls terminal differentiation during adipogenesis. PMID: 23285057
    4. PARP-1 as a novel KLF8-binding and -regulating protein and provided new insights into the mechanisms underlying the regulation of KLF8 nuclear localization, stability, and functions. PMID: 21518760
    5. These results identified the KLF8 activation domain located between residues 101-260 where the well-conserved Q118 and Q248 are essential for recruiting p300 and PCAF to activate target gene transcription. PMID: 20107328
    6. sumoylation of KLF8 negatively regulates its transcriptional activity and cellular functions PMID: 16617055
    7. Klf8 is repressed by Klf3 and activated by Klf1 in vivo. PMID: 18687676
    8. KLF8 has two N terminal regulatory regions, one surrounding S165 and K171 and the other being two tandem ZFs, which are critical for the regulation of KLF8 nuclear localization and its cellular functions PMID: 19488069

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Sp1 C2H2-type zinc-finger protein family
  • 數據庫鏈接：


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