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  4. Recombinant Mouse Intraflagellar transport protein 27 homolog (Ift27)

Recombinant Mouse Intraflagellar transport protein 27 homolog (Ift27)

  • 中文名稱：
    小鼠Ift27重組蛋白
  • 貨號：
    CSB-YP861511MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Ift27重組蛋白
  • 貨號：
    CSB-EP861511MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Ift27重組蛋白
  • 貨號：
    CSB-EP861511MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Ift27重組蛋白
  • 貨號：
    CSB-BP861511MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Ift27重組蛋白
  • 貨號：
    CSB-MP861511MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ift27
  • Uniprot No.：
  • 別名：
    Ift27; Rabl4; Rayl; Intraflagellar transport protein 27 homolog; Putative GTP-binding protein RAY-like; Rab-like protein 4
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-186
  • 氨基酸序列
    MVKLAAKCIL AGDPAVGKTA LVQMFRSDGT HFQKNYTLTT GVDLVVKTVP VLDTNDSVEL FIFDSAGKEL FSEMLDKLWE NPNVLCLVYD VTNEQSFISC TKWLEKVRSQ TSGISLPGVL VGTKTDLAGR QTVDSAQAQV WALSQGLEFF ETSVKEMDNY EAPFHCLAKQ FYQLYREKVD IFHTLV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6. Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling. Forms a subcomplex within the IFT complex B with IFT25. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation.
  • 基因功能參考文獻：
    1. Findings suggest that like IFT25, IFT27, even though not required for ciliogenesis in somatic cells, is essential for sperm flagella formation, sperm function, and male fertility in mice. IFT25 and IFT27 control sperm formation/function through many common mechanisms, but IFT25 has additional roles beyond IFT27. PMID: 28964737
    2. The study implicates Ift27 in basal mechanosensation. PMID: 27231153
    3. this study demonstrated an essential role of Ift27 in mediating hedgehog signaling during follicular keratinocyte proliferation and differentiation but not the differentiation program of the interfollicular epidermis. PMID: 26023097
    4. Lztfl1 accumulate to high levels in Ift27 mutant cilia. PMID: 25446516
  • 亞細胞定位：
    Cell projection, cilium. Cytoplasm. Cell projection, cilium, flagellum.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 組織特異性：
    Expressed predominantly in the testis (at protein level). Co-localizes with RABL2/RABL2A in the midpiece of elongated spermatids within the testis (at protein level).
  • 數據庫鏈接：


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