Recombinant Mouse Group 10 secretory phospholipase A2 (Pla2g10)

中文名稱：

小鼠Pla2g10重組蛋白
貨號：

CSB-BP882554MO
規格：

￥3168
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Pla2g10
Uniprot No.：

Q9QXX3
別名：

Group X secretory phospholipase A2 (GX sPLA2) (sPLA2-X) (Phosphatidylcholine 2-acylhydrolase 10)
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

Baculovirus
分子量：

17.8 kDa
表達區域：

29-151aa
氨基酸序列

GLLELAGTLDCVGPRSPMAYMNYGCYCGLGGHGEPRDAIDWCCYHHDCCYSRAQDAGCSPKLDRYPWKCMDHHILCGPAENKCQELLCRCDEELAYCLAGTEYHLKYLFFPSILCEKDSPKCN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

28-38 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids with preference for phosphatidylcholines and phosphatidylglycerols over phosphatidylethanolamines. Preferentially releases sn-2 omega-6 and omega-3 polyunsaturated fatty acyl (PUFA) chains over saturated fatty acyls. Contributes to phospholipid remodeling of very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells. Efficiently hydrolyzes and inactivates PAF, a potent lipid mediator present in oxidized LDL. May act in an autocrine and paracrine manner. Secreted by lung epithelium, targets membrane phospholipids of infiltrating eosinophils, releasing arachidonate and boosting eicosanoid and cysteinyl leukotriene synthesis involved in airway inflammatory response. Secreted by gut epithelium, hydrolyzes dietary and biliary phosphatidylcholines in the gastrointestinal lumen, thereby regulating adipogenesis and body weight. Plays a stem cell regulator role in colon epithelium. Within intracellular compartment, mediates Paneth-like cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ISC). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates the Wnt signaling pathway in ISCs and tissue regeneration. May participate in hair follicle morphogenesis by regulating phosphatidylethanolamines metabolism at the outermost epithelial layer and facilitating melanin synthesis. By generating lysophosphatidylcholines (LPCs) at sperm acrosome controls sperm cell capacitation, acrosome reaction and overall fertility. May promote neurite outgrowth in neuron fibers involved in nociception. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. In pulmonary epithelium, may contribute to host defense response against adenoviral infection. Prevents adenovirus entry into host cells by hydrolyzing host cell plasma membrane, releasing C16:0 LPCs that inhibit virus-mediated membrane fusion and viral infection. Likely prevents adenoviral entry into the endosomes of host cells. May play a role in maturation and activation of innate immune cells including macrophages, group 2 innate lymphoid cells and mast cells.
基因功能參考文獻：
1. secretory PLA2s have important functions as genetic modifiers of inflammation and colon cancer. PMID: 27292189
2. PLA2G10 releases omega-3 polyunsaturated fatty acids, suppresses colitis, and promotes sperm fertility. PMID: 26828067
3. Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2beta (iPLA2beta) and Group X Secreted Phospholipase A2 (sPLA2). PMID: 26655718
4. GX sPLA2 negatively regulated pancreatic insulin secretion by augmenting COX-2-dependent PGE2 production. PMID: 25122761
5. Mouse PLA2GX has a unique property of improving fertilization outcome among other secreted phospholipase A2 isoforms. PMID: 24287291
6. Data indicate that experimental allergic bronchitis (EAB) in was associated with increased expression of sPLA2, specifically sPLA2gX, in the lungs. PMID: 24204651
7. Authors propose that activation of GX-sPLA2 during H1N1pdm infection is an early step of pulmonary inflammation and its inhibition increases adaptive immunity and improves survival. PMID: 24725934
8. Pla2G10 limits the development of atherosclerosis in LDL receptor-null mice. PMID: 23349189
9. the use of a highly potent indole-based inhibitor of hGX-sPLA(2), RO061606 (which is ineffective against mGX-sPLA(2)), to assess the potential utility of GX-sPLA(2) blockade as a therapeutic intervention in asthma. PMID: 21652694
10. sPLA(2)-X in neutrophils plays a pathogenic role in abdominal aortic aneurysms in a mouse model. PMID: 21984544
11. sPLA2 plays a new role for fertilization by selecting a sperm subpopulation based on its effect on sperm motility. PMID: 21792918
12. Group X secretory phospholipase A2 promotes macrophage inflammatory responses by altering cellular cholesterol homeostasis and lipid raft content. PMID: 21622863
13. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function. PMID: 21266581
14. the spatiotemporal expression of sPLA(2)-X in hair follicles, the presence of skin-specific machinery leading to sPLA(2)-X activation, a functional link of sPLA(2)-X with hair follicle homeostasis. PMID: 21266583
15. GX sPLA(2) promotes Ang II-induced pathological responses leading to abdominal aortic aneurysm formation PMID: 20833395
16. hydrolytic products generated by GX sPLA(2) negatively regulate adipogenesis, possibly by suppressing LXR activation PMID: 20585029
17. The overexpression of GX sPLA(2) significantly reduced ABCA1 and ABCG1 expression in J774 macrophage-like cells, whereas GX sPLA(2) deficiency in peritoneal macrophages was associated with enhanced expression. PMID: 20844270
18. Group X phospholipase A2 is released during sperm acrosome reaction and controls fertility outcome in mice PMID: 20424324
19. LDL modification by GXPLA2 [GXPLA2] PMID: 12021277
20. group X secreted phospholipase A2 is expressed in neural cells and has neuritogenic action PMID: 15781456
21. sPLA2-X induces potent arachidonic acid release without activation of cytosolic phospholipase A2 alpha. PMID: 15789617
22. group V, but not group X, secreted phospholipase A2 has a role in lung dysfunction PMID: 17008322
23. direct attention to group X-secreted phospholipase A2 as a novel therapeutic target for asthma PMID: 17403936
24. Myocardial ischemia/reperfusion injury was attenuated in sPLA(2)-X(-/-) mice partly through the suppression of neutrophil cytotoxic activities PMID: 18506007
25. secretory phospholipase A2 group X enhances anti-inflammatory responses, promotes lipid accumulation, and contributes to aberrant lung pathology PMID: 18511424
26. Enhanced protein expression in mammalian cells using engineered SUMO fusions: example of secreted PLA2-X. PMID: 18539905
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亞細胞定位：

Secreted. Lysosome. Cytoplasmic vesicle, secretory vesicle, acrosome.
蛋白家族：

Phospholipase A2 family
組織特異性：

Expressed at high levels in testis and the gastrointestinal tract including stomach and colon. Expressed at lower levels in other tissues including small intestine, uterus, oviduct, lung, thymus, spleen and brain. Expressed in Paneth-like secretory epithe
數據庫鏈接：

KEGG: mmu:26565

STRING: 10090.ENSMUSP00000023364

UniGene: Mm.4214