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  4. Recombinant Mouse E3 ubiquitin-protein ligase TRIM50 (Trim50)

Recombinant Mouse E3 ubiquitin-protein ligase TRIM50 (Trim50)

  • 中文名稱：
    小鼠Trim50重組蛋白
  • 貨號：
    CSB-YP772186MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Trim50重組蛋白
  • 貨號：
    CSB-EP772186MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Trim50重組蛋白
  • 貨號：
    CSB-EP772186MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Trim50重組蛋白
  • 貨號：
    CSB-BP772186MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Trim50重組蛋白
  • 貨號：
    CSB-MP772186MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Trim50
  • Uniprot No.：
  • 別名：
    Trim50; E3 ubiquitin-protein ligase TRIM50; EC 2.3.2.27; RING-type E3 ubiquitin transferase TRIM50; Tripartite motif-containing protein 50
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-483
  • 氨基酸序列
    MAWRLTVPEL QDQLQCPICL EVFKEPLMLQ CGHSYCKDCL DNLSQHLDSE LCCPVCRQSV DCSSSPPNVS LARVIDALRL PGDIEPTVCV HHRNPLSLFC EKDQEFICGL CGLLGSHQHH RVTPVSTVYS RMKEELAGRI SELKEEHRNV EEHIGKLVNN RTRIINESDV FSWVIRREFQ ELHHLVDEEK ARCLEGLEGH TRGLVASLDM QLEQAQGTQE RLAQAEQVLE QFGNESHHEF IRFHSVASRA EVQQARPLEG VFSPISFKPA LHQADIKLTV WKRLFRKVLP APASLKLDPA TAHPLLELSK GNTVVHCGLL AQRRASQPER FDYSTCVLAS KGFSWGRHYW EVVVGSKSDW RLGVIKGTAS RKGKLNKSPE HGVWLIGLKE GRVYEAFGCP RLPLPVAGHP HRIGVYLHYE QGELTFFDAD RPDDLRTLYT FQADFQGKLY PILDTCWHER GSNSLPMVLP PPSGPGHFTL GQV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.
  • 基因功能參考文獻：
    1. TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. PMID: 29604308
    2. TRIM50 protein regulates vesicular trafficking for acid secretion in gastric parietal cells PMID: 22872646
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRIM/RBCC family
  • 組織特異性：
    Expressed in the stomach.
  • 數據庫鏈接：

    KEGG: mmu:215061

    UniGene: Mm.261834



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