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  4. Recombinant Mouse DNA polymerase alpha subunit B (Pola2)

Recombinant Mouse DNA polymerase alpha subunit B (Pola2)

  • 中文名稱：
    小鼠Pola2重組蛋白
  • 貨號：
    CSB-YP018301MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Pola2重組蛋白
  • 貨號：
    CSB-EP018301MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Pola2重組蛋白
  • 貨號：
    CSB-EP018301MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Pola2重組蛋白
  • 貨號：
    CSB-BP018301MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Pola2重組蛋白
  • 貨號：
    CSB-MP018301MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Pola2DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-600
  • 氨基酸序列
    MAVSTQQLAE ELQIFGLDYE DSLLEKLAEL CVLYRQTEDG MVSELIAFCT SAGKTCLTVD ILNSFEYEVL NKKLSKAWHS ASKDSGHAGT RDIVSIQELI EAEEEEETLL SSYTTPSKGP LKRVSSTPET PLTKRSVAAR SPRQLLSPSS FSPSATPSQK YTSRTNRGEV VTTFGSAQGL SWSGRGGSGS VSLKVVGDPE PLTGSYKAMF QQLMGVREVL TSKIEELGSE LKEHHKIEAF TPLLVPAQEP VILLGQIGCD SNGKLNSKSV ILEGDQEHSY GAQIPVDLSE LKEYSLFPGQ VVIMEGFNTT GRRLTATKLY EGVPLPFYQP TEEEGASEQT MVVVACGPYT TSDSITYDPL LDLIAIINRD QPDVCILFGP FLDAKHEQVE NCKLTSPFED VFKQCLRTVI EGTRSSGSHL VFVPSLRDVH HEPVYPQPPF TFSELSREDK KRVQFVSEPC SLSINGVMFG LTSTDLLFHI GAEEIFSSSG TSDRFSRVLK HILTQRSYYP LYPPHEDMAI DYENFYTYAQ LPVTPDVFIV PSELRYFVKD IFGCVCVNPG RLTKGQVGGT FGRLYLRRQP KAMDGGGRQG LSVAAQVVRI
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.
  • 基因功能參考文獻(xiàn)：
    1. nonphosphorylated p68 inhibited the stimulation of pol-alpha activity by hyperphosphorylated retinoblastoma protein, suggesting that p68 might impede the association of ppRb with p180 [Dna polymerase alpha p68] PMID: 16935576
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase alpha subunit B family
  • 數(shù)據(jù)庫鏈接：


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