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  4. Recombinant Mouse DNA-directed RNA polymerase I subunit RPA49 (Polr1e)

Recombinant Mouse DNA-directed RNA polymerase I subunit RPA49 (Polr1e)

  • 中文名稱：
    小鼠Polr1e重組蛋白
  • 貨號：
    CSB-YP814303MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Polr1e重組蛋白
  • 貨號：
    CSB-EP814303MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Polr1e重組蛋白
  • 貨號：
    CSB-EP814303MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Polr1e重組蛋白
  • 貨號：
    CSB-BP814303MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Polr1e重組蛋白
  • 貨號：
    CSB-MP814303MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Polr1e
  • Uniprot No.：
  • 別名：
    Polr1e; Paf53; Praf1; DNA-directed RNA polymerase I subunit RPA49; RNA polymerase I subunit A49; DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-482
  • 氨基酸序列
    MATLESPGMD DQAGDTETEA LQSARWLYCG EPDDRQKAVL VQFSNGKLQN PGDMRFTLYN STDLVNPRQR SHRIVAAETD RLSYVGNNFG TGALKCNALC RHFVGILNKT SGQMEVYDAE LFNMQPLFAG MGTEVIKLGG QHLYLLAFCQ PSKNLAEAGD LLLSRHRQGH CIAVLLDDDA IEREPPLENQ NKTFRDKLDS CIEAFGSTKQ KRSLNSRRMN KVGSESLNLS VAKAAESIID TKGVNALVSD AMQDDLQDGV LYLPPCYADA AKPEDVYRFE DILSPAEYDA LESPSEAFRK VTSEDILKMI EENSHCSYVI EMLKSLPIDE VHRNRQARSI WFLDALIRFR AQKVIKGKRA LGPGIPHIIN TKLLKQFTCL TYNNGRLQNL ISSSMRAKIT SYAIILALHI NNFQVDLTAL QKDLKLSEKR MIEIAKAMRL KISKQKVSLA DGREESHRLG TLSVPLPPAQ NSDRQSKRRK MN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
  • 基因功能參考文獻：
    1. DNA sequencing demonstrated that in the absence of ectopic PAF53 expression, cells demonstrated unique means of surviving; including recombination or the utilization of alternative reading frames. We never observed a clone in which one PAF53 gene is expressed, unless there was also ectopic expression In the absence of ectopic gene expression, the gene products of both endogenous genes were expressed, irrespective of wheth PMID: 28042089
    2. yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric complexes interact through species-specific sequence elements that appear to assume similar structural motifs. PMID: 22849406
  • 亞細胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Eukaryotic RPA49/POLR1E RNA polymerase subunit family
  • 數據庫鏈接：

    KEGG: mmu:64424

    UniGene: Mm.282932



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