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Recombinant Mouse Alpha-hemoglobin-stabilizing protein (Ahsp)

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  • 中文名稱:
    Recombinant Mouse Alpha-hemoglobin-stabilizing protein (Ahsp)
  • 貨號:
    CSB-EP875156MO
  • 規格:
    ¥1536
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    Ahsp
  • Uniprot No.:
  • 別名:
    Erythroid differentiation-related factor;Erythroid-associated factor
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    18.7 kDa
  • 表達區域:
    1-102aa
  • 氨基酸序列
    MAPFQSNKDLISTGIKEFNVLLDQQVFDDPLISEEDMVIVVHDWVNLYTNYYKKLVHGEQEEQDRAMTEFQQELSTLGSQFLAKYRTFLKSKEPPSNTLPSS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    C-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation.
  • 基因功能參考文獻:
    1. role of alpha-hemoglobin stabilizing protein in hemoglobin assembly PMID: 22287545
    2. Endogenous AHSP is not limiting for alpha-globin detoxification in beta-thalassemic mice. PMID: 20815047
    3. An abundant erythroid protein that stabilizes free alpha-haemoglobin. PMID: 12066189
    4. activin A does not appear to affect the exocrine or endocrine components of the pancreas, but promotes differentiation of pancreatic tissue into intestine via a Shh-dependent mechanism PMID: 15054113
    5. Loss of AHSP impairs erythropoiesis and exacerbates beta-thalassemia. PMID: 15545996
    6. These results support the hypothesis that EKLF acts as a transcription factor and a chromatin modulator for the AHSP and beta-globin genes and indicate that EKLF may play similar roles for other erythroid genes. PMID: 16705186
    7. developmental stage- and differentiation state-specific regulation of the nuclear transport of EKLF might be one of the steps necessary for the switch-on of the mammalian adult beta globin gene transcription PMID: 17242208
    8. AHSP also acts as a molecular chaperone to stabilize nascent alpha-globin for hemoglobin A assembly PMID: 17607360
    9. The expression of AHSP in a subclone of murine erythroleukemia cells in relationship to prion protein on the cell surface is reported. PMID: 18828445

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  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    AHSP family
  • 組織特異性:
    Expressed in spleen, bone marrow, and blood, with highest levels in bone marrow.
  • 數據庫鏈接:

    KEGG: mmu:170812

    UniGene: Mm.423023



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