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  4. Recombinant Mouse ATP-dependent DNA helicase PIF1 (Pif1)

Recombinant Mouse ATP-dependent DNA helicase PIF1 (Pif1)

  • 中文名稱：
    小鼠Pif1重組蛋白
  • 貨號：
    CSB-YP767304MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Pif1重組蛋白
  • 貨號：
    CSB-EP767304MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Pif1重組蛋白
  • 貨號：
    CSB-EP767304MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Pif1重組蛋白
  • 貨號：
    CSB-BP767304MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Pif1重組蛋白
  • 貨號：
    CSB-MP767304MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pif1
  • Uniprot No.：
  • 別名：
    Pif1; ATP-dependent DNA helicase PIF1; EC 3.6.4.12; DNA repair and recombination helicase PIF1; Pif1/Rrm3 DNA helicase-like protein
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-650
  • 氨基酸序列
    MRSGLCTPAE ALEMPSSTEA ATDECDDAEL RCRVAVEELS PGGQPRKRQA LRAAELSLGR NERRELMLRL QAPGPTGRPR CFPLRAVRLF TRFAATGRST LRLPTDGVPG AGSVQLLLSD CPPERLRRFL RTLRLKLAVA PGPGPASARA QLLGPRPRDF VTISPVQPEE LQRAAATKAP DSALEKRPME SQTSTEAPRW PLPVKKLRMP STKPKLSEEQ AAVLRMVLKG QSIFFTGSAG TGKSYLLKHI LGSLPPTGTV ATASTGVAAC HIGGTTLHAF AGIGSGQAPL AQCMALANRP GVRQGWLNCQ RLVIDEISMV EADFFDKLEA VARAVRQQKK PFGGIQLIIC GDFLQLPPVT KGSQQPQFCF QAKSWRRCVP VILELTEVWR QADQTFISLL QAVRLGRCSD EVTRQLRATA AHKVGRDGIV ATRLCTHQDD VALTNEKWLK ALPGDVHSFE AIDSDPELSR TLDAQCPVSR VLQLKLGAQV MLVKNLAVSR GLVNGARGVV VGFESEGRGL PRVRFLCGIT EVIRTDRWTV QVTGGQYLSR QQLPLQLAWA ISIHKSQGMS LDCVEISLGR VFASGQAYVA LSRARSLQGL RVLDFDPTVV RCDSRVLHFY ATLRQGRGLS LESQDDEEAN SDLENMDPNL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.
  • 基因功能參考文獻：
    1. pif1-/- animals develop a mitochondrial myopathy with respiratory chain deficiency. PMID: 26923168
    2. murine cells have evolved mechanisms to ensure the functional redundancy of Pif1 or Nbs1 in the regulation of chromosome healing. PMID: 21945094
    3. Murine telomere homeostasis or genetic stability does not depend on mPif1. PMID: 17130244
  • 亞細胞定位：
    Nucleus.; [Isoform 3]: Mitochondrion.
  • 蛋白家族：
    Helicase family, PIF1 subfamily
  • 數據庫鏈接：


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