Recombinant Methanosarcina barkeri tRNA-splicing endonuclease (endA)

中文名稱：

Recombinant Methanosarcina barkeri tRNA-splicing endonuclease(endA)
貨號：

CSB-YP672762MSM
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Methanosarcina barkeri tRNA-splicing endonuclease(endA)
貨號：

CSB-EP672762MSM
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Methanosarcina barkeri tRNA-splicing endonuclease(endA)
貨號：

CSB-EP672762MSM-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Methanosarcina barkeri tRNA-splicing endonuclease(endA)
貨號：

CSB-BP672762MSM
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Methanosarcina barkeri tRNA-splicing endonuclease(endA)
貨號：

CSB-MP672762MSM
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

endA
Uniprot No.：

Q46FK9
別名：

endA; Mbar_A0349tRNA-splicing endonuclease; EC 4.6.1.16; tRNA-intron endonuclease
種屬：

Methanosarcina barkeri (strain Fusaro / DSM 804)
蛋白長度：

full length protein
表達區域：

1-350
氨基酸序列

MKTQLKGDRV LAEKEAVAEF YKTGYFGRPK GEGLELSLVE AAFLLSRGKL EIELEGTMLD FRYFFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR VYPRGSHPGK SAARIFVHVQ SERQLLPVKL LQDSVISAEN VHKQFILAVV DEESDLTFYE VKTAVPKGEM TEPYPAIKTD ATFLEDRVIA WDAQASETLY KRGFYGKMLD SERLQLSLVE SLYLFSRGII TVRDRKGKVF SFDEFIEKAS EIESFFLRKY SAYKNLRDSG HVVKTGFKFG TNFRVYRKVE SIEKIPHSEY LVNAIPADFE FRLSVMSGAV RLANSVRKKM LFAVEKDEEI EYLDISRTKM
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
蛋白家族：

TRNA-intron endonuclease family, Archaeal long subfamily
數據庫鏈接：

KEGG: mba:Mbar_A0349

STRING: 269797.Mbar_A0349