在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Measles virus Fusion glycoprotein F0 (F), partial

In Stock
  • 貨號:
    CSB-EP301654MCQ
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    F
  • Uniprot No.:
  • 種屬:
    Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    55.3 kDa
  • 表達區域:
    24-494aa
  • 氨基酸序列
    QIHWGNLSKIGVVGIGSASYKVMTRSSHQSLVIKLMPNITLLNNCTRVEIAEYRRLLRTVLEPIRDALNAMTQNIRPVQSVASSRRHKRFAGVVLAGAALGVATAAQITAGIALHQSMLNSQAIDNLRASLETTNQAIEAIRQAGQEMILAVQGVQDYINNELIPSMNQLSCDLIGQKLGLKLLRYYTEILSLFGPSLRDPISAEISIQALSYALGGDINKVLEKLGYSGGDLLGILESRGIKARITHVDTESYFIVLSIAYPTLSEIKGVIVHRLEGVSYNIGSQEWYTTVPKYVATQGYLISNFDESSCTFMPEGTVCSQNALYPMSPLLQECLRGSTKSCARTLVSGSFGNRFILSQGNLIANCASILCKCYTTGTIINQDPDKILTYIAADHCPVVEVNGVTIQVGSRRYPDAVYLHRIDLGPPISLERLDVGTNLGNAIAKLEDAKELLESSDQILRSMKGLSSTS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (F protein) probably interacts with H at the virion surface. Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes. Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis.
  • 基因功能參考文獻:
    1. Cell-to-Cell Measles Virus Spread between Human Neurons Is Dependent on Hemagglutinin and Hyperfusogenic Fusion Protein. PMID: 29298883
    2. L454W F mutant is activated independently of H or the cell receptor that enabled the efficient spread in the central nervous system. PMID: 25670774
    3. Acquisition of enhanced fusion activity through substitutions in the extracellular domain of the F protein may be crucial for measles virus to the extensive spread in the central nervous system and development of subacute sclerosing panencephalitis. PMID: 25520515
    4. Complementation of F mutants with a monomeric, fusion-inactive F variant enriched the F oligomers for heterotrimers containing a single disulfide bond, without affecting fusion complementation profiles compared with standard F protein. PMID: 25157143
    5. F maturation prepares for complex separation after triggering, and the H head domains in prereceptor-bound conformation prevent premature stalk rearrangements and F activation. PMID: 25392208
    6. Thermodynamically stabilized by the N465H substitution, the F protein required elevated temperature as high as 40 degrees C to promote cell-cell fusion, whereas all five DIII mutations caused destabilization of the F protein PMID: 25479085
    7. Authors demonstrate that the measles virus H stalk represents the effector domain for measles virus F triggering. PMID: 23966411
    8. Taken together, these findings reveal that the morbillivirus H protein must lower the activation energy barrier of metastable prefusion F for fusion triggering. PMID: 23077316
    9. The F genes of measles virus isolated in china had no significant genetic variation. PMID: 20084883
    10. The F genes of measles virus in China during 1999-2003 had no sig-nificant changes. PMID: 20077667
    11. residues located in the head domain of the F trimer and the HR-B region contribute jointly to controlling F conformational stability PMID: 16415028
    12. findings show that both M (a fraction of which is modified by ubiquitination) & F proteins individually promote formation of virus-like particles, but they do not act in synergy; we propose that M & F act separately in particle morphogenesis and release PMID: 17374768
    13. These findings suggest a common molecular mechanism and a key role of the F protein for syncytium formation in cells expressing an unidentified third receptor for MV. PMID: 17825451
    14. Tyrosine-based targeting motifs in the H and F glycoproteins play a crucial role for MV replication and spread within lymphocytes, the main target cells of acute MV infection. PMID: 18272759
    15. The partitioning of F, CD46 and CD55 molecules in different membrane microdomains could account for the ability of F to escape complement regulation by the CD55 and CD46 regulators. PMID: 18455798
    16. Alanine-scanning mutagenesis revealed that an F protein with a single mutation of a central TM region leucine (L507A) was more fusogenic than the unmodified F protein while retaining similar kinetics of proteolytic processing. PMID: 18786999
    17. The H-protein and the F-protein mediates the virus cell entry. PMID: 19198562
    18. findings argue against specific protein-protein contacts between the H head & F head domains; support a docking model characterized by short-range contacts between prefusion F head & attachment protein stalk, possibly involving H residues 111, 114 & 118 PMID: 19656895

    顯示更多

    收起更多

  • 亞細胞定位:
    Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein.
  • 蛋白家族:
    Paramyxoviruses fusion glycoprotein family
  • 數據庫鏈接:

    KEGG: vg:1489800



主站蜘蛛池模板: 免费无码又爽又刺激高潮| 国产md视频一区二区三区| 亚洲美女高清无水av| 国产成人精品.视频| 欧美国产精品久久久乱码| 国内精品伊人久久久久网站| 国产小呦泬泬99精品| 精品无人区卡一卡二卡三乱码| 久久精品亚洲国产av老鸭网| 天堂资源在线www在线观看| 潮喷无码正在播放| 777午夜福利理论电影网| 亚洲 日韩 欧美 成人 在线观看| 亚洲精品欧美精品日韩精品| 日本一卡2卡3卡4卡免费乱码网站| 国产精品免费福利久久| 中日韩高清无专码区2021| 国产亚洲精品久久久久9999| 性色av一区二区三区咪爱四虎| 华人少妇被黑人粗大的猛烈进 | 99久久国产综合精品麻豆| 激情综合色综合久久综合| 337p日本大胆欧久久| 精品一卡2卡三卡4卡免费网站| 国产欠欠欠18一区二区| 成人试看30分钟免费视频| 久久se精品一区二区三区| 亚洲欧美综合区自拍另类| 成人欧美一区二区三区在线观看| 综合无码成人aⅴ视频在线观看 | 亚洲成a∧人片在线观看无码| 一区二区三区av高清免费波多| 中文字幕无码精品亚洲35| 先锋影音xfyy5566男人资源| 99久久亚洲综合精品成人| 中文字幕被公侵犯的漂亮人妻 | 吃奶摸下的激烈视频| 精品无人乱码高清| 久久精品国产一区二区三区不卡| 免费观看激色视频网站| 亚洲国产欧美日韩精品一区二区三区|