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Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial

  • 中文名稱:
    Recombinant Mannheimia haemolytica Leukotoxin(lktA),partial
  • 貨號(hào):
    CSB-YP314743ESE
  • 規(guī)格:
    ¥2616
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    lktA
  • Uniprot No.:
  • 別名:
    lktA; Leukotoxin; Lkt
  • 種屬:
    Mannheimia haemolytica (Pasteurella haemolytica)
  • 蛋白長度:
    Partial
  • 來源:
    Yeast
  • 分子量:
    28.0kDa
  • 表達(dá)區(qū)域:
    715-953aa
  • 氨基酸序列
    IGTSHNDIFKGSKFNDAFNGGDGVDTIDGNDGNDRLFGGKGDDIIDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRQGDGNDSITESEGNDKLSFSDSNLKDLTFEKVNHHLVITNTKQEKVTIQNWFREAEFAKTIQNYVATRDDKIEEIIGQNGERITSKQVDELIEKGNGKIAQSELTKVVDNYQLLKYSRDASNSLDKLISSASAFTSSNDSRNVLASPTSMLDPSLSSIQFARAA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述:

    The N-terminal 6xHis tag was fused to the gene fragment corresponding to the 715-953aa of the Mannheimia haemolytica lktA protein and then was cloned into an expression vector. The expression vector was transfected into the yeast cells for expression. The generated product was purified and separated to obtain the recombinant Mannheimia haemolytica lktA protein. Its purity is higher than 90%. This recombinant lktA protein showed an apparent molecular weight of about 30 kDa under SDS-PAGE condition.

    The specified functions of lktA protein in diseases is not well understood. Some studies suggested that lktA production by Fusobacterium necrophorum strains in relation to severity of liver abscesses in cattle. Several researches suggested that lktA is related to the tumors. For example, it has been identified that lktA induces ATP expulsion through pannexin-1 channels and subsequent cell death in malignant lymphocytes.

  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca(2+) and lysis of the host cell. This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It has also weak hemolytic activity.
  • 亞細(xì)胞定位:
    Secreted. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    RTX prokaryotic toxin (TC 1.C.11) family


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