Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial

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中文名稱：

單核增生李斯特菌血清1/2ainlA重組蛋白
貨號：

CSB-EP338647LPY(M)
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

inlA
Uniprot No.：

P0DJM0
種屬：

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
蛋白長度：

Partial
來源：

E.coli
分子量：

48.9 kDa
表達區域：

32-414aa(S192N,Y369S)
氨基酸序列

NAQAATITQDTPINQIFTDTALAEKMKTVLGKTNVTDTVSQTDLDQVTTLQADRLGIKSIDGVEYLNNLTQINFSNNQLTDITPLKNLTKLVDILMNNNQIADITPLANLTNLTGLTLFNNQITDIDPLKNLTNLNRLELSSNTISDISALSGLTSLQQLNFGNQVTDLKPLANLTTLERLDISSNKVSDISVLAKLTNLESLIATNNQISDITPLGILTNLDELSLNGNQLKDIGTLASLTNLTDLDLANNQISNLAPLSGLTKLTELKLGANQISNISPLAGLTALTNLELNENQLEDISPISNLKNLTYLTLYFNNISDISPVSSLTKLQRLFFSNNKVSDVSSLANLTNINWLSAGHNQISDLTPLANLTRITQLGLND
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA.
基因功能參考文獻：
1. Deletion of either one or both proteins leads to a similarly decreased invasion, suggesting an interdependent function of InlA and InlB during invasion of choroid plexus epithelial cells. PMID: 23376167
2. About 40% of Listeria monocytogenes isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes PMID: 23317868
3. A novel single amino acid change in inlA enhanced virulence by the oral route in the murine model. PMID: 21144051
4. Listeria monocytogenes internalin interacts with E-cadherin [review] PMID: 20066101
5. L. monocytogenes subtypes are common among U.S. food isolates but rare among human listeriosis isolates carry inlA mutations. PMID: 16332872
6. Differential expression levels of inlA and inlB possibly play a role in the virulence capacities of Listeria monocytogenes strains. PMID: 16751490
7. Sequence analyses of inlA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated inlA which indicated that these strains could be fully virulent based on the size of this protein. PMID: 17566579
8. the essential and interdependent roles of InlA and InlB in fetoplacental listeriosis PMID: 18806773
9. analysis of the internalin multigene family in Listeria PMID: 18840511
10. truncated and/or proteolytically cleaved InlA are likely involved in the biofilm enhancement. PMID: 19309566
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亞細胞定位：

Secreted, cell wall; Peptidoglycan-anchor. Secreted.
數據庫鏈接：

KEGG: lmo:lmo0433

STRING: 169963.lmo0433