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  4. Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA), partial

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA), partial

  • 中文名稱：
    單核增生李斯特菌血清1/2ainlA重組蛋白
  • 貨號：
    CSB-YP338647LPY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    單核增生李斯特菌血清1/2ainlA重組蛋白
  • 貨號：
    CSB-EP338647LPY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    單核增生李斯特菌血清1/2ainlA重組蛋白
  • 貨號：
    CSB-BP338647LPY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    單核增生李斯特菌血清1/2ainlA重組蛋白
  • 貨號：
    CSB-MP338647LPY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    inlA
  • Uniprot No.：
  • 種屬：
    Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA.
  • 基因功能參考文獻：
    1. Deletion of either one or both proteins leads to a similarly decreased invasion, suggesting an interdependent function of InlA and InlB during invasion of choroid plexus epithelial cells. PMID: 23376167
    2. About 40% of Listeria monocytogenes isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes PMID: 23317868
    3. A novel single amino acid change in inlA enhanced virulence by the oral route in the murine model. PMID: 21144051
    4. Listeria monocytogenes internalin interacts with E-cadherin [review] PMID: 20066101
    5. L. monocytogenes subtypes are common among U.S. food isolates but rare among human listeriosis isolates carry inlA mutations. PMID: 16332872
    6. Differential expression levels of inlA and inlB possibly play a role in the virulence capacities of Listeria monocytogenes strains. PMID: 16751490
    7. Sequence analyses of inlA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated inlA which indicated that these strains could be fully virulent based on the size of this protein. PMID: 17566579
    8. the essential and interdependent roles of InlA and InlB in fetoplacental listeriosis PMID: 18806773
    9. analysis of the internalin multigene family in Listeria PMID: 18840511
    10. truncated and/or proteolytically cleaved InlA are likely involved in the biofilm enhancement. PMID: 19309566

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  • 亞細胞定位：
    Secreted, cell wall; Peptidoglycan-anchor. Secreted.
  • 數(shù)據(jù)庫鏈接：

    KEGG: lmo:lmo0433

    STRING: 169963.lmo0433



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