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  4. Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein (gag), partial

Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein (gag), partial

  • 中文名稱：
    Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein(gag) ,partial
  • 貨號：
    CSB-YP322411HLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein(gag) ,partial
  • 貨號：
    CSB-EP322411HLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein(gag) ,partial
  • 貨號：
    CSB-EP322411HLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein(gag) ,partial
  • 貨號：
    CSB-BP322411HLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein(gag) ,partial
  • 貨號：
    CSB-MP322411HLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    gag; Gag polyprotein; Pr55Gag) [Cleaved into: Matrix protein p17; MA); Capsid protein p24; CA); Spacer peptide 1; SP1; p2); Nucleocapsid protein p7; NC); Spacer peptide 2; SP2; p1); p6-gag]
  • 種屬：
    Human immunodeficiency virus type 2 subtype A (isolate BEN) (HIV-2)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).; Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus. Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA.; Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry. Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating. On the other hand, interactions with PDZD8 or CYPA stabilize the capsid.; Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly.; Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.
  • 基因功能參考文獻：
    1. The differential requirement for adaptor protein complexes confirmed that HIV-1 and HIV-2 Gag have distinct cellular trafficking pathways, and that HIV-2 particles may be more susceptible to degradation prior to release. PMID: 27392064
    2. These data on HIV-2 indicate that the role of the matrix domain in the nucleic acid chaperone activity of Gag differs not only between, but also within, retroviral genera. PMID: 26987314
    3. These data show that infectious HIV-2 cannot bear the replacement of the reverse transcriptase Gln294 Pro, as it results in substantially reduced HIV-2 replication and fast reversions to the wild-type Gln294 virus. PMID: 25965791
    4. This showed that V111I changed the flexibility of the 110-to-115 loop region, which may affect deoxynucleoside triphosphate (dNTP) binding and polymerase activity. PMID: 25355888
    5. HIV-2 genome dimerization is required for the correct processing of Gag. PMID: 22419802
    6. The N-terminal and C-terminal domains of the integrase were highly conserved. PMID: 21114823
    7. analysis of the secondary active domain of HIV-2 NCp8 PMID: 17511966
    8. The results show that all modifications of HIV-2 RT Gln294 lead to an increase of the RNase H activity of the enzyme relative to the wild-type. PMID: 18625228
    9. C1-C4 region of HIV2 SU glycoprotein impairs utilization of the CCR5 and CXCR4 for viral entry. PMID: 19200444
    10. These results suggest that the splicing of both the 5'UTR intron and the C-box element have key roles in regulation of HIV-2 gag translation in vitro and in vivo. PMID: 19559029

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  • 亞細胞定位：
    [Gag polyprotein]: Host cell membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p17]: Virion membrane; Lipid-anchor. Host nucleus. Host cytoplasm.; [Capsid protein p24]: Virion.; [Nucleocapsid protein p7]: Virion.
  • 蛋白家族：
    Primate lentivirus group gag polyprotein family


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