在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human Replication protein A 32 kDa subunit (RPA2), partial

Recombinant Human Replication protein A 32 kDa subunit (RPA2), partial

In Stock
  • 中文名稱：
    人RPA2重組蛋白
  • 貨號：
    CSB-EP020089HU1
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    60S acidic ribosomal protein P1; AA409079; AI325195; AU020965; ik:tdsubc_2g1; M(2)21C; MGC137236; OTTHUMP00000004008 ; p32; p34; RCJMB04_6d17 replication protein A2; 32kDa ; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2; 32kDa ; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32 ; RPA34; RpLP1; RpP2; xx:tdsubc_2g1; zgc:109822
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    55.9kDa
  • 表達(dá)區(qū)域：
    1-267aa
  • 氨基酸序列
    MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKST
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal GST-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
  • 基因功能參考文獻：
    1. RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
    2. Single point mutations in the RPA32 subunit of RPA that abolish interaction with RFWD3 also inhibit interstrand crossling repair, demonstrating that RPA-mediated RFWD3 recruitment to stalled replication forks is important for ICL repair. PMID: 28575657
    3. The authors show that Vpr can form a trimolecular complex with UNG2 and RPA32 and the positive effect of UNG2 and RPA32 on the reverse transcription process leading to optimal virus replication and dissemination between the primary target cells of HIV-1. PMID: 27068393
    4. RPA32 phosphorylation regulates replication arrest, recombination, late origin firing, and mitotic catastrophe PMID: 24819595
    5. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. PMID: 25113031
    6. Conserved motifs are required for RPA32 binding the the N-terminus of SMARCAL1. PMID: 24910198
    7. study reports the characterization of the RPA32C-SMARCAL1 interface at the molecular level; implications of results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks PMID: 24730652
    8. study concludes RPA2 expression is translationally regulated via internal ribosome entry site and by eIF3a and that this regulation is partly accountable for cellular response to DNA damage and survival. PMID: 23393223
    9. this study has explored the role of RPA32 phosphorylation at CDK and ATR sites and propose that phosphorylation of the RPA32 subunit is dispensable for checkpoint activation induced by replication stress with aphidicolin. PMID: 23047005
    10. 4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2 PMID: 22684010
    11. Data show that the R88C variant impairs binding of the R88C variant impairs binding of uracil-DNA glycosylase UNG2 to replication protein A RPA2. PMID: 22521144
    12. Replication protein A1, replication protein A2, and cyclins D2 and D3 seem to have a parallel role in the promotion of cell cycle in astrocytic tumors being implicated in the malignant progression of these neoplasms. PMID: 21496876
    13. RPA2 up-regulation may be involved in the growth and/or survival of BRCA1 tumor cells and useful in immunohistochemical discrimination of triple-negative BRCA1 tumors. PMID: 21137066
    14. At the subunit level, 13 proteins out of 30 examined may interact with RPA2. PMID: 20679368
    15. data suggest that RPA2 hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologus recombination PMID: 20130019
    16. Data suggest that PP4-mediated dephosphorylation of RPA2 is necessary for an efficient DNA-damage response. PMID: 20154705
    17. RPA32, critical for cell proliferation and maintenance of genome stability, are markedly down-regulated, Data hypothesized that their DNA-related functions could be partially limited in TRAIL-resistant HL-60 cells. PMID: 19834905
    18. Phosphorylation of the RPA2 subunit is observed after exposure of cells to ionizing radiation (IR) and other DNA-damaging agents, which implicates the modified protein in the regulation of DNA replication after DNA damage or in DNA repair. PMID: 11731442
    19. RPA2 binds to menin and has a role in multiple endocrine neoplasia PMID: 12509449
    20. C-terminal domain of hRPA32 subunit (RPA32C) facilitates initiation of SV40 replication. PMID: 15793585
    21. in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication PMID: 17035231
    22. Determination at single-nucleotide resolution the relative positions of the single-stranded DNA with interacting intrinsic tryptophans of RPA32. PMID: 17583916
    23. RPA phosphorylation facilitates chromosomal DNA repair. PMID: 17928296
    24. RPA32 is extensively phosphorylated after the induction of EBV lytic replication. Rad51 and RPA32 are necessary for the completion of EBV lytic infection. PMID: 19386720
    25. The N-terminus of RPA1 and phosphorylation of RPA2 regulate RPA interactions with the MRE11-RAD50-NBS1 (MRN) complex and are important in the response to DNA damage. PMID: 19586055
    26. mitotic phosphorylation of RPA2 starts at the onset of mitosis, and dephosphorylation occurs during late cytokinesis. PMID: 19671522

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Nucleus. Nucleus, PML body.
  • 蛋白家族：
    Replication factor A protein 2 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 10290

    OMIM: 179836

    KEGG: hsa:6118

    STRING: 9606.ENSP00000363021

    UniGene: Hs.79411



主站蜘蛛池模板： 熟睡人妻被讨厌的公侵犯深田咏美| 亚洲人毛茸茸bbxx| 久久精品国产精品亚洲毛片| 女人高潮流白浆视频| 亚洲欧洲日产国码韩国| 首页 国产 亚洲 丝袜图片区| 人妻av无码专区久久| 女人与拘做受全过程免费视频| 午夜免费福利小电影| av国産精品毛片一区二区网站| 亚洲成av人片天堂网无码】| 少妇高潮一区二区三区99| 大胸美女被吃奶爽死视频| 欧美男生射精高潮视频网站| 日本孕妇潮喷高潮视频| 亚洲精品久久一区二区无卡| 国产精品久久久久久久久久妞妞 | 亚洲欧洲日产国码无码久久99| 一本色道无码道在线观看| 国产精品自在线一区| 亚洲日韩一区二区| 色悠久久久久久久综合网伊人| 欧美亚洲综合成人专区| 日本少妇寂寞少妇aaa| 欧美做爰性生交视频| 亚洲性无码av中文字幕| 亚洲国产精品成人综合色| 蜜桃av麻豆av果冻传媒| 236宅宅理论片免费| 天天躁日日躁狠狠久久| 日本一卡2卡3卡4卡免费乱码网站 日本无遮挡吸乳呻吟视频 | 人人爽人人爽人人爽| 看曰本女人大战黑人视频| 成年奭片免费观看视频天天看| 亚洲中文字幕日产无码2020| 少妇高潮一区二区三区99| 深夜国产一区二区三区在线看| 免费无码在线播放av| 性色av免费网站| 久久亚洲一区二区三区四区五区| 国产男女猛烈视频在线观看|