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  4. Recombinant Human Protein phosphatase 1E (PPM1E)

Recombinant Human Protein phosphatase 1E (PPM1E)

  • 中文名稱：
    人PPM1E重組蛋白
  • 貨號：
    CSB-YP820184HU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PPM1E重組蛋白
  • 貨號：
    CSB-EP820184HU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PPM1E重組蛋白
  • 貨號：
    CSB-EP820184HU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PPM1E重組蛋白
  • 貨號：
    CSB-BP820184HU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PPM1E重組蛋白
  • 貨號：
    CSB-MP820184HU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PPM1E
  • Uniprot No.：
  • 別名：
    Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKN; CaMKP-N; CaMKP-nucleus; KIAA1072; Nuclear calmodulin dependent protein kinase phosphatase; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; POPX1; PP2CH; Ppm1e; PPM1E_HUMAN; Protein phosphatase 1E; Protein phosphatase Mg2+/Mn2+ dependent 1E
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-764
  • 氨基酸序列
    MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPEPEPE PEPESEPEPE PELVEAEAAE ASVEEPGEEA ATVAATEEGD QEQDPEPEEE AAVEGEEEEE GAATAAAAPG HSAVPPPPPQ LPPLPPLPRP LSERITPRPL SERITREEVE GESLDLCLQQ LYKYNCPSFL AAALARATSD EVLQSDLSAH YIPKETDGTE GTVEIETVKL ARSVFSKLHE ICCSWVKDFP LRRRPQLYYE TSIHAIKNMR RKMEDKHVCI PDFNMLFNLE DQEEQAYFAV FDGHGGVDAA IYASIHLHVN LVRQEMFPHD PAEALCRAFR VTDERFVQKA ARESLRCGTT GVVTFIRGNM LHVAWVGDSQ VMLVRKGQAV ELMKPHKPDR EDEKQRIEAL GGCVVWFGAW RVNGSLSVSR AIGDAEHKPY ICGDADSAST VLDGTEDYLI LACDGFYDTV NPDEAVKVVS DHLKENNGDS SMVAHKLVAS ARDAGSSDNI TVIVVFLRDM NKAVNVSEES DWTENSFQGG QEDGGDDKEN HGECKRPWPQ HQCSAPADLG YDGRVDSFTD RTSLSPGSQI NVLEDPGYLD LTQIEASKPH SAQFLLPVEM FGPGAPKKAN LINELMMEKK SVQSSLPEWS GAGEFPTAFN LGSTGEQIYR MQSLSPVCSG LENEQFKSPG NRVSRLSHLR HHYSKKWHRF RFNPKFYSFL SAQEPSHKIG TSLSSLTGSG KRNRIRSSLP WRQNSWKGYS ENMRKLRKTH DIPCPDLPWS YKIE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2.
  • 基因功能參考文獻：
    1. Ppm1E upregulation in human gastric cancer is important for cell proliferation, possible via regulating AMPK-mTOR signaling. PMID: 28423719
    2. miR-135b-5p expression downregulates Ppm1e to activate AMPK signaling, which inhibits LPS-induced TNFalpha production via suppressing ROS production and NFkappaB activation. PMID: 27793001
    3. our results suggest that miR-135b-induced Ppm1e silence induces AMPK activation to inhibit osteoblastoma cell proliferation. PMID: 28460435
    4. Data show that miR-135b selectively targets the AMPK phosphatase Ppm1e. PMID: 27661114
    5. The Ppm1E contributes to dephosphorylation of AMP-activated protein kinase. PMID: 20801214
    6. The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    PP2C family
  • 數據庫鏈接：

    HGNC: 19322

    STRING: 9606.ENSP00000312411

    UniGene: Hs.245044



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