Recombinant Human Prostaglandin-H2 D-isomerase (PTGDS)

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中文名稱：

Recombinant Human Prostaglandin-H2 D-isomerase (PTGDS)
貨號：

CSB-EP018969HU(M1)
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

PTGDS
Uniprot No.：

P41222
別名：

Beta-trace protein;Cerebrin-28;Glutathione-independent PGD synthase;Lipocalin-type prostaglandin-D synthase;L-PGDS;Prostaglandin-D2 synthase;PGD2 synthase;PGDS;PGDS2
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

24.5 kDa
表達(dá)區(qū)域：

23-190aa(176-185:TIVFLPQTDK→Missing)
氨基酸序列

APEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDCMTEQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
基因功能參考文獻(xiàn)：
1. High lipocalin-type prostaglandin D synthase expression is associated with Spontaneous intracranial hypotension. PMID: 29621631
2. Measurement of serum BTP can be a reliable tool for detecting kidney function in neonates. PMID: 29421771
3. Thus, our results suggest decreased NK cell-mediated eosinophil regulation, possibly through an increased level of PGD2, as a previously unrecognized link between PG dysregulation and eosinophilic inflammation in CRS. PMID: 27271931
4. These observations suggest that tumor cell-derived inflammatory cytokines increase L-PGDS expression and subsequent PGD2 production in the tumor endothelial cells (ECs). This PGD2 acts as a negative regulator of the tumorigenic changes in tumor ECs. PMID: 29124765
5. The cut off value for betaTP in the diagnosis and follow-up of cerebrospinal fluid leaks should be modified depending on the type of secretion (sample type), for a better diagnostic accuracy PMID: 27614217
6. Serum levels of beta trace protein and beta 2 microglobulin can be used to predict residual kidney function in hemodialysis patients. PMID: 26924065
7. BTP and B2M levels are less affected than serum by amputation, and should be considered for future study of GFR estimation PMID: 26800100
8. Hematopoietic prostaglandin D synthase defines a proeosinophilic pathogenic effector human T(H)2 cell subpopulation with enhanced function PMID: 26431580
9. levels of L-PGDS in cervicovaginal secretions of pregnant women at different stages of parturition correlate with preterm birth. PMID: 25964109
10. Given that uPGDS levels fall after treatment of LN, uPGDS may be used to monitor the efficacy of therapy. It can also differentiate patients with active nephritis and active non renal lupus. PMID: 26211517
11. PTGDS mRNA expression was down-regulated in rapid-cycling bipolar disorder patients in a euthymic, depressive, and manic/hypomanic state compared with healthy control subjects. PMID: 25522430
12. Expression of MR and prostaglandin D2 synthase is strongly correlated in adipose tissues from obese patients. PMID: 25966493
13. involved in pathogenesis of androgenetic alopecia [review] PMID: 24521203
14. These data indicate that scalp is spatially programmed via mast cell prostaglandin D-synthase distribution in a manner reminiscent of the pattern seen in androgenetic alopecia. PMID: 24438498
15. These results suggest that L-PGDS acted as a scavenger of biliverdin, which is a molecule not found in normal CSF. PMID: 25005874
16. Among NSTE-ACS patients, BTP and CysC were superior to conventional renal parameters for predicting MB, and improved clinical stratification for hemorrhagic risk. PMID: 23698027
17. Structural and dynamic insights into substrate binding and catalysis of human lipocalin prostaglandin D synthase. PMID: 23526831
18. betaTP, beta2M, CysC, and creatinine differ in their associations with demographic and clinical factors, suggesting variation in their non-glomerular filtration rate determinants. PMID: 23335043
19. All-cause and cardiovascular mortality are strongly associated with beta-trace protein marker levels and beta-microglobulin in a representative sample of US adults. PMID: 23518194
20. The results we obtained from mice led to our investigation of PTGDS in 29 human cryptorchid patients but we failed to find any mutation that supported an involvement of this gene in human testicular descent. PMID: 23076868
21. no overall evidence of an association between wireless phone use and serum concentrations of beta-trace protein PMID: 22989106
22. Elevated plasma level of beta-trace protein associated with all cause of death in patients with acute coronary syndrome. PMID: 22818840
23. The serum level of beta-trace protein is an independent predictor of death and cardiovascular disease mortality in incident hemodialysis patients. PMID: 22745274
24. Low PTGDS expression is associated with testis cancer. PMID: 22960220
25. L-PGDS expression may contribute to the restricted proliferation of epidermal melanocytes, but conversely its overexpression may reflect the dysregulated proliferation of melanoma cells. PMID: 22299829
26. The gene coding for PTGDS was found to be more expressed in patients with attention deficit hyperactivity disorder (ADHD)relative to patients with bipolar disorder indicating a possible link with the differential etiology of ADHD. PMID: 22370065
27. Data suggest that L-PGDS binds small lipophilic ligands with both high-affinity and low-affinity interactions; molecular models are proposed from studies that include binding of hemin, biliverdin, and bilirubin. PMID: 22677050
28. Ascites and pleural effusion contain high concentrations of beta-TP that exceed the levels in corresponding plasma PMID: 21501068
29. Urinary PGDS, not ZA2G, may serve as a biomarker for active LN and upon validation in larger studies, may become the non-invasive test to evaluate the disease activity in future management of LN. PMID: 22498882
30. NM 000954 NM 000954 PMID: 22342541
31. these results demonstrate that L-PGDS protected against neuronal cell death by scavenging reactive oxygen species without losing its ligand-binding function PMID: 22248185
32. Beta-trace protein can be used as an alternative diagnostic tool to detect moderate or severe glomerular filtrate rate reduction in patients after liver transplantation. PMID: 21745310
33. two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in epicardial adipose tissue compared to mediastinal and subcutaneous adipose tissue PMID: 21603615
34. Lipocalin-type prostaglandin D synthase is associated with coronary vasospasm and vasomotor reactivity in response to acetylcholine PMID: 21325722
35. Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma. PMID: 21271676
36. RBP-4, lipocalin-2 and L-PGDS do not regulate insulin sensitivity in healthy men. Rather their expression seemed to reflect inflammatory activity and were inversely correlated with alcohol intake and serum HDL levels. PMID: 21104585
37. Report the presence of L-PGDS in the COX-2-expressing cells in the mucosa of active ulcerative colitis patients in parallel with disease activity. PMID: 21163901
38. It may be feasible to test for perilymphatic fluid fistula using PTGDS in samples from the tympanic cavity. PMID: 21192373
39. Met64 seems to function as a kinetic clamp, pushing the thiol group of Cys65 close to the site of nucleophilic attack during catalysis. PMID: 20667974
40. Using RT-PCR we demonstrated that L-PGDS gene expression decreased proportionately with tumor progression in lung cancer. PMID: 20144489
41. L-PGDS may fine-tune the retinoic acid signalling in melanocytes. PMID: 20403807
42. This study included 62 persons aged 18-30 years and cell phone exposure. EMF emissions may down-regulate the synthesis of beta-trace protein. PMID: 20596612
43. protein levels in nasal fluids can serve for diagnosis of cerebrospinal fluid leak PMID: 19958607
44. This beta-trace protein based formula was found to estimate GFR with reasonable precision PMID: 19949816
45. The expression of H-PGDS in human dendritic cells (DCs) and the regulatory mechanisms by which DCs produce prostaglandin D2, is demonstrated. PMID: 20008150
46. As the CSF is in contact with axons and mitochondria of the optic nerve, we postulate that a change in the concentration of CSF protein such as L-PGDS could exercise a harmful effect on these structures. PMID: 19598000
47. Pronounced eosinophilia and Th2 cytokine release in human lipocalin-type prostaglandin D synthase transgenic mice PMID: 11751991
48. Study performed in patients with kidney function ranging from normal to advanced renal failure suggests that serum beta-trace protein is an indicator of impaired glomerular filtration rate. PMID: 12900000
49. The circadian lipocalin-type PGDS pattern and its suppression by total sleep deprivation indicate an interaction of the prostaglandin D system and human sleep regulation. PMID: 15453544
50. Shear stress induces l-PGDS expression by transcriptional activation through the AP-1 binding site. PMID: 15718494
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亞細(xì)胞定位：

Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted. Note=Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
組織特異性：

Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prosta
數(shù)據(jù)庫鏈接：

HGNC: 9592

OMIM: 176803

KEGG: hsa:5730

STRING: 9606.ENSP00000360687

UniGene: Hs.446429