Recombinant Human Prolyl endopeptidase (PREP), partial

中文名稱：

人PREP重組蛋白
貨號(hào)：

CSB-EP018664HU
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

PREP
Uniprot No.：

P48147
種屬：

Homo sapiens (Human)
蛋白長度：

partial
來源：

E.coli
分子量：

88.0 kDa
表達(dá)區(qū)域：

2-710aa
氨基酸序列

LSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
基因功能參考文獻(xiàn)：
1. These findings provide evidence for newly-discovered roles for PREP in mechanisms regulating cellular plasticity through NCAM and PSA-NCAM. PMID: 27566163
2. Study shows that the expression of POP increases with hepatocyte steatosis suggesting an important role in hepatocytes steatosis and possibly NAFLD. PMID: 27760195
3. It was found that human PREP behaves identically to the porcine homolog, displaying a double bell-shaped pH profile and a pH-dependent solvent kinetic isotope effect of the kcat/Km, features that set it apart from the related exopeptidase dipeptidyl peptidase IV (DPP IV). PMID: 28062644
4. TLR4 activation releases prolyl endopeptidase containing exosomes from airway epithelial cells. PMID: 26222144
5. This study found that circulating PREP activity was substantially reduced in all cirrhotic patients but those changes did not have evident correlation to hepatic encephalopathy. PMID: 26420028
6. PEP was significantly and positively associated with delinquent, aggressive, externalizing and internalizing behavior subscales. PMID: 26165750
7. PREP redox inactivation is due to oxidation of cysteine residues and consequent oligomerization through intermolecular disulfide bonds. PMID: 25236746
8. Data indicate that SAXS analysis showed that presequence protease (hPreP) open states and substrate binding dynamics. PMID: 24931469
9. The determination of PEP activity in the plasma may be a safe, minimally invasive and inexpensive way to define the aggressiveness of CRC in daily practice. PMID: 24465166
10. first demonstration of colocalization of PREP and pathological proteins in the human brain, supporting the view that, at least in spatial terms, PREP could be associated with pathogenesis of neurodegenerative diseases PMID: 23562579
11. These results indicate that POP may be a positive regulator of cell cycle progression by regulating the exit from and/or reentry into the cell cycle by KATO III cells. PMID: 24269815
12. interaction between prolyl oligopeptidase and glyceraldehyde-3-phosphate dehydrogenase is required for cytosine arabinoside-induced glyceraldehyde-3-phosphate dehydrogenase nuclear translocation and cell death PMID: 23348613
13. Oxidative stress and the levels of endogenous plasma prolyl oligopeptidase (PREP) inhibitor alpha-2-macroglobulin (alpha2M) decrease PREP activity in multiple sclerosis patients. PMID: 23643808
14. PREP may be associated with secretory processes as well as in reproduction. A more abundant expression of PREP in malignant than benign tumors suggests that PREP may be associated with expansion and metastasis of tumors. PMID: 22740343
15. analysis of the substrate preferences of two post-proline cleaving endopeptidases, prolyl oligopeptidase and fibroblast activation protein alpha PMID: 22750443
16. PREP is a regulatory target and a regulatory element in cell signalling. This is the first report of a direct influence of a cell signalling molecule, retinoic acid, on PREP expression. PMID: 21487212
17. Molecular modeling and docking based approaches were used to unravel questions like differences in ligand binding affinities in three POP species (porcine, human and A. thaliana). PMID: 22132071
18. These results indicate that POP is a part of the machinery that controls the cell cycle. PMID: 21620802
19. Results indicate that there is no strong and direct interaction between POP and GAP43 at physiological conditions. PMID: 20869470
20. The constitutive expression of PREP mRNA in chronic lymphocytic leukemia was demonstrated. PMID: 20534982
21. Cytosolic Prolyl endopeptidase activity significantly increased in clear cell renal cell carcinoma, urothelial carcinoma of the renal pelvis and head and neck squamous cell carcinoma. PMID: 20362629
22. Plasma levels of PREP activity as well as those of their endogenous inhibitor are suggested as biomarkers of inflammation and oxidative stress in multiple sclerosis PMID: 20370893
23. The high activity of prolyl endopeptidase in the human cortex suggests that prolyl endopeptidase could play a role in cortical functions. Activity in humans is highest in the frontal lobe and cytosolic fractions. PMID: 11792464
24. The activities of pyroglutamyl peptidase I and prolyl endopeptidase in necrozoospermia were found to be higher in the corresponding soluble and particulate sperm fractions, respectively, with respect to those measured in normozoospermic semen PMID: 15380924
25. primary structure, recombinant expression and homology modelling of brain prolyl oligopeptidase PMID: 15838896
26. Mainly localized to perinuclear space and associated with microtubulin cytoskeleton in human neuroblastoma and glioma. Novel functions in axonal transport and/or protein secretion. PEP inhibitors may be useful in variety of related clinical conditions. PMID: 16092940
27. data demonstrate for the first time prolyl endopeptidase turnover of humanin by a limited post-cysteine as well as post-proline proteolysis resulting in the inactivation of this potentially apoptosis-related factor PMID: 16700513
28. Results describe the distribution of immunoreactive prolyl oligopeptidase in human and rat brain. PMID: 17401647
29. The results rule out a causative role of POP in the pathogenesis of CD and strongly suggest that other peptidases are needed to eliminate gliadin-derived, immunoactive and toxic peptides larger than 33-mer, which is a POP inhibitor. PMID: 17454876
30. Data reveal for the first time the presence of a new side opening in prolyl oligopeptidase that was not observed in any of the crystallographic structures described to date. PMID: 19782684
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Peptidase S9A family
數(shù)據(jù)庫鏈接：

HGNC: 9358

OMIM: 600400

KEGG: hsa:5550

STRING: 9606.ENSP00000358106

UniGene: Hs.436564