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  4. Recombinant Human Metal-response element-binding transcription factor 2 (MTF2)

Recombinant Human Metal-response element-binding transcription factor 2 (MTF2)

  • 中文名稱：
    人MTF2重組蛋白
  • 貨號：
    CSB-YP896720HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人MTF2重組蛋白
  • 貨號：
    CSB-EP896720HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人MTF2重組蛋白
  • 貨號：
    CSB-EP896720HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人MTF2重組蛋白
  • 貨號：
    CSB-BP896720HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人MTF2重組蛋白
  • 貨號：
    CSB-MP896720HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MTF2
  • Uniprot No.：
  • 別名：
    MTF2; PCL2; Metal-response element-binding transcription factor 2; Metal regulatory transcription factor 2; Metal-response element DNA-binding protein M96; Polycomb-like protein 2; hPCl2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-593
  • 氨基酸序列
    MRDSTGAGNS LVHKRSPLRR NQKTPTSLTK LSLQDGHKAK KPACKFEEGQ DVLARWSDGL FYLGTIKKIN ILKQSCFIIF EDSSKSWVLW KDIQTGATGS GEMVCTICQE EYSEAPNEMV ICDKCGQGYH QLCHTPHIDC SVIDSDEKWL CRQCVFATTT KRGGALKKGP NAKALQVMKQ TLPYSVADLE WDAGHKTNVQ QCYCYCGGPG DWYLKMLQCC KCKQWFHEAC VQCLQKPMLF GDRFYTFICS VCSSGPEYLK RLPLQWVDIA HLCLYNLSVI HKKKYFDSEL ELMTYINENW DRLHPGELAD TPKSERYEHV LEALNDYKTM FMSGKEIKKK KHLFGLRIRV PPVPPNVAFK AEKEPEGTSH EFKIKGRKAS KPISDSREVS NGIEKKGKKK SVGRPPGPYT RKMIQKTAEP LLDKESISEN PTLDLPCSIG RTEGTAHSSN TSDVDFTGAS SAKETTSSSI SRHYGLSDSR KRTRTGRSWP AAIPHLRRRR GRLPRRALQT QNSEIVKDDE GKEDYQFDEL NTEILNNLAD QELQLNHLKN SITSYFGAAG RIACGEKYRV LARRVTLDGK VQYLVEWEGA TAS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Can you send me some informations of Recombinant Human Metal-response element-binding transcription factor 2 (MTF2), Yeast, cat.nr. : CSB-YP896720HU.
I would also like to know if the protein is folded properly

A:
Thanks for your inquiry.
Recombinant Human Metal-response element-binding transcription factor 2(MTF2)
Express region:1-593aa；Full lenth protein.
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
Target Protein Sequence:

MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKINILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDCSVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPGDWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVIHKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRVPPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEPLLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWPAAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAGRIACGEKYRVLARRVTLDGKVQYLVEWEGATAS


As to the fold, we haven't done the related identification, but the protein produced by the yeast system is theoretically capable of folding correctly and is a soluble protein, with glycosylation, phosphorylation and acylation post-translational modification, the buffer we provided does not contain any denaturant ingredients, theoretically correct folding should be no problem.
Q:

I am looking for a recombinant Mtf2 protein for gel shift assay and I am interested in your product CSB-YP896720HU. Before ordering I would however need to know the exact buffer composition it is shipped in and the concentration of the protein.

A:
Thanks for your inquiry.
The shipping buffer is: Tris-based buffer，pH 8.0，50% glycerol
The concentration is arround 0.1-2mg/ml. Because the concentration will be different in different lot.

靶點詳情

  • 功能：
    Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter.
  • 基因功能參考文獻：
    1. the F61L/S86F mutant of MTF2 Tudor-PHD1 was able to bind to H3K36me3 as strong as the PHF1 Tudor bound to this PTM . We concluded that the hydrophobic patch plays an essential role in binding of these Tudors to methylated chromatin PMID: 25923537
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Polycomblike family
  • 數據庫鏈接：

    HGNC: 29535

    OMIM: 609882

    KEGG: hsa:22823

    STRING: 9606.ENSP00000359321

    UniGene: Hs.31016



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