Recombinant Human MAP kinase-interacting serine/threonine-protein kinase 1 (MKNK1)

^{In Stock}

中文名稱：

人MKNK1重組蛋白
貨號(hào)：

CSB-EP861139HU
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

MKNK1
Uniprot No.：

Q9BUB5
別名：

MAP kinase signal-integrating kinase 1;MAPK signal-integrating kinase 1;Mnk1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

58.2 kDa
表達(dá)區(qū)域：

1-465aa
氨基酸序列

MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
基因功能參考文獻(xiàn)：
1. MNK1 is involved in regulating both IRES- and cap-dependent viral mRNA translation. [review] PMID: 29864503
2. High expression of MNK1 is frequent in HCC tissues, which promotes tumor proliferation and invasion, and is correlated with a poor overall survival. PMID: 29576605
3. High MNK1 expression in epithelial ovarian cancer tissues indicates poor clinical outcomes PMID: 28332091
4. these data show that NDRG1 is regulated by the oncogenic MAP kinase-interacting kinase pathway, a target for cancer therapy PMID: 28545025
5. MKNK1 polymorphism was associated with treatment response in metastatic colorectal cancer. PMID: 29045529
6. Elevated levels of p-Mnk1, p-eIF4E and p-p70S6K proteins are associated with tumor recurrence and poor prognosis in astrocytomas. Overexpression of p-eIF4E and co-expression of p-Mnk1, p-eIF4E and p-p70S6K proteins could be used as novel independent poor prognostic biomarkers for patients with astrocytomas. PMID: 27900644
7. MNK-1 controls chemokine secretion and proliferation in human airway smooth muscle cells. PMID: 27418099
8. MNK1 encodes a Ser/Thr protein kinase that interacts with extracellular signal-regulated kinase 1 and p38 mitogen-activated protein kinase, a pathway that is involved in Blood Pressure regulation through norepinephrine and angiotensin II. PMID: 27271309
9. Data show that galeterone (gal) and VNPT55 inhibit migration and invasion of prostate cancer cells, possibly by down-regulating protein expression via antagonizing the Mnk1/2-eIF4E axis. PMID: 27618366
10. data suggest a physiological role for MNK1a-Ser(353) phosphorylation in regulation of the MNK1a kinase, which correlates with increased eIF4E phosphorylation in vitro and in vivo. PMID: 27413184
11. Data suggest MNK1/MNK2 stimulate mRNA translation but only of mRNA containing both 5-prime-terminal cap and hairpin duplex; this stimulation involves up-regulation of phosphorylation/mRNA un-winding activity of eIF4E (via decreased binding to eIF4G). PMID: 26668315
12. Simultaneous targeting of androgen receptor and MNK1 by novel retinamides inhibits growth of human prostate cancer cell lines. PMID: 25605250
13. MNK1 and MNK2 inhibition ablates eIF4E1 phosphorylation and concurrently enhances eIF4E3 expression in diffuse large B-cell lymphoma. PMID: 25403230
14. Data show that interferon-gamma regulated the metabolism and mRNA translation of macrophages by targeting the kinases mTORC1 and MNK1/2, both of which converge on the selective regulator of translation initiation eukaryotic initiation factor-4E (eIF4E). PMID: 26147685
15. Data suggest that a combined pharmacologic inhibition of mTORC1 and Mnk1/2 kinases offers a therapeutic opportunity in blast crisis-chronic myeloid leukemia (BC-CML). PMID: 25527453
16. Authors show that MNK regulates SRPK via mTOR and AKT. PMID: 25187540
17. ERK1/2 signal induced MNK catalytic activity enabled enterovirus type 1 internal ribosomal entry site-mediated translation/host cell cytotoxicity through negative regulation of the Ser/Arg (SR)-rich protein kinase (SRPK). PMID: 25187541
18. These data indicate multiple myeloma cells exploit the MNK/eIF-4E pathway for selective mRNA translation without enhancing global translation and risking ER stress. PMID: 24714040
19. High expression of p-Mnk1 and p-eIF4E might be novel valuable biomarkers to predict poor prognosis of nasopharyngeal carcinoma. PMID: 24551240
20. rapalog-activated MNK1 signaling promotes glioma growth through regulation of 4EBP1; there is a molecular cross-talk between the mTORC1 and MNK1 pathways PMID: 24401275
21. These findings provide evidence for key and essential roles of the Mnk kinase pathway in the generation of the antineoplastic effects of type I IFNs in Jak2V617F-dependent myeloproliferative neoplasms. PMID: 23814052
22. MNK1, which participates in translational control in several cell types, is activated in response to physiological neutrophil agonists (LPS, TNF-alpha) in the cytoplasmic and nuclear compartments. PMID: 23401599
23. TGFbeta induces signaling involving PI3kinase-dependent Mnk-1-mediated phosphorylation of eIF4E at Ser-209 to facilitate mesangial cell hypertrophy. A role for dissociation of 4EBP-1-eIF4E complex for Mnk-1-mediated phosphorylation of eIF4E. PMID: 23359369
24. Our findings identify the MNK-eIF4E axis as a specific and critical regulator of blast crisis self-renewal, and suggest that pharmacologic inhibition of the MNK kinases may be therapeutically useful in BC chronic myeloid leukemia. PMID: 23737503
25. Chemical inhibition or siRNA knockdown of MKNK1 significantly impaired entry of genotype 1a hepatitis C virus in Huh-7 cells but had only minimal impact on viral RNA replication or cell proliferation and viability. PMID: 23365451
26. MNK1 kinase activity is required for abscission. PMID: 22454512
27. Resistance to trastuzumab was observed in tumor cells with elevated MNK1 expression, furthermore, inhibition of RSK1 restored sensitivity to resistant cells. PMID: 22249268
28. These data suggest that MNK1 regulates the phosphorylation and the subcellular distribution of hnRNP A1 and that MNK1 may play a role in the induction of senescence PMID: 22227431
29. These findings indicate that the combined inhibition of mTORC1 and MNK may prove beneficial in the treatment of cutaneous T-cell lymphoma and other malignancies. PMID: 21949767
30. Data show that PKCalpha activation elicits a cascade of orchestrated phosphorylation events that may modulate eIF4G1 structure and control interaction with the eIF4E kinase, Mnk1. PMID: 21576361
31. Findings offer insights into how MNK1 pathways control translation of cancer-related mRNAs including SMAD2, a key component of the TGF-beta signaling pathway. PMID: 21406405
32. siRNA-mediated Mnk1/2 knockdown results in partial reversal of the suppressive effects of IFNgamma on human CD34+-derived myeloid (CFU-GM) and erythroid (BFU-E) progenitors. PMID: 21149447
33. Data suggest that a proportion of breast cancers could be sensitive to inhibiting MNK kinase activity, and that the presence of phosphorylated eIF4E could provide a biomarker for the identification of responsive tumours. PMID: 20686366
34. Adenovirus 100K protein blocks cellular protein synthesis by coopting eIF4G and cap-initiation complexes and displacing or blocking binding by Mnk1, which occurs only on preassembled complexes, resulting in dephosphorylation of eIF4E. PMID: 15220445
35. Mnk1 phosphorylation by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk PMID: 15234964
36. role for MNK1 in the AML fusion protein-associated differentiation block PMID: 15516979
37. Interleukins 2 and 15 regulate Ets1 expression via ERK1/2 and MNK1 in human natural killer cells PMID: 15563472
38. Data show that Mnk1 suppression decreases eukaryotic initiation factor 4F phosphorylation without causing any change in global protein synthesis rate and cell proliferation. PMID: 15581611
39. Mnk1-mediated serine phosphorylation of Spry2 constitutes a regulatory mechanism to extend the temporal range of Spry2 activity. PMID: 16479008
40. Data show that inorganic phosphate controls cell growth by activating ERK1/2 cascades and by facilitating the translocation of Mnk1 from cytosol into nucleus through an Akt-mediated MEK pathway. PMID: 16763222
41. The activity of MKNK1 was characterized. PMID: 17590453
42. mTOR inhibition increases eIF4E phosphorylation through a PI3K-dependent and Mnk-mediated mechanism. PMID: 17724079
43. A conserved phenylalanine residue in an Mnk-specific insert is playing a key role in governing the ease with which Mnk1a can be phosphorylated. PMID: 19650764
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亞細(xì)胞定位：

[Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family
組織特異性：

Ubiquitous.
數(shù)據(jù)庫鏈接：

HGNC: 7110

OMIM: 606724

KEGG: hsa:8569

STRING: 9606.ENSP00000361014

UniGene: Hs.371594