Recombinant Human MAP kinase-activating death domain protein (MADD), partial

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中文名稱：

Recombinant Human MAP kinase-activating death domain protein (MADD), partial
貨號：

CSB-EP819910HU
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

MADD
Uniprot No.：

Q8WXG6
別名：

Differentially expressed in normal and neoplastic cells;Insulinoma glucagonoma clone 20;Rab3 GDP/GTP exchange factor;RabGEF;Rab3 GDP/GTP exchange protein;Rab3GEP
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

18.4 kDa
表達區域：

1409-1506aa
氨基酸序列

YSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family. Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion. Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. Involved in upregulating a post-docking step of synaptic exocytosis in central synapses. Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals. Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2. May link TNFRSF1A with MAP kinase activation. May be involved in the regulation of TNFA-induced apoptosis.
基因功能參考文獻：
1. The risk of coronary disease and ischemic stroke associated with multiple polymorphisms and haplotypes of MADD and FOLH1 in Han Chinese patients are reported in association with alcohol consumption. PMID: 27070640
2. miR-3151 silencing by DNA methylation protected chronic lymphocytic leukemia cells from apoptosis by over-expression of its direct targets MADD and PIK3R2, constitutive activation of MEK/ERK and PI3K/AKT signaling , and over-expression of MCL1. PMID: 26517243
3. Taken together, our data reveal that PTEN can convey the death signal by preventing MADD phosphorylation by Akt. PMID: 24038283
4. These results suggest that MADD may be a potential therapeutic target for lung adenocarcinoma therapy involving the TRAIL-induced apoptosis pathway PMID: 23443411
5. MADD knockdown resulted in enhanced spontaneous apoptosis in human breast cancer cell lines PMID: 23457619
6. We analyzed DENN/MADD/IG20 (DMI), the complex of four splice variants in Alzheimer's disease. PMID: 22678883
7. SNP rs2290149, located in a genetic cluster of MYBPC3 and MADD gene, was found to be associated with diastolic heart failure. PMID: 22529996
8. Data show that ehe expression of MAPK-activating death domain protein (MADD) increases obviously in lung adenocarcinoma, and MADD can promote survival of lung adenocarcinoma cells by inhibiting apoptosis. PMID: 22482404
9. The SNP in ADCY5 is implicated in defective proinsulin-to-insulin conversion and previous findings on the role of a genetic variant in MADD on proinsulin-to-insulin conversion, were confirmed. PMID: 21887289
10. Data show that SNPs from MADD, PROX1, and SLC30A8 were associated with type 2 diabetes. PMID: 21103350
11. Endogenous MADD is phosphorylated at three highly conserved sites by Akt, and only the phosphorylated MADD can directly interact with the TRAIL receptor DR4 thereby preventing Fas-associated death domain recruitment. PMID: 20484047
12. Denn is intimately involved in anti-apoptotic and cell survival processes in cancer cells PMID: 12410563
13. IG20 overexpression enhanced the combined apoptotic effects of TRAIL and gamma-irradiation on HeLa cells. PMID: 14695193
14. IG20 suppresses tumor cell survival and enhances susceptibility to apoptosis and cancer therapy. PMID: 14716293
15. DENN has anti-apoptotic and cell survival roles in tumor cells PMID: 14735464
16. IG20 (MADD splice variant-5) enhances TRAIL-induced apoptosis by increasing recruitment of FADD and caspase-8 to the death-inducing signaling complex. PMID: 15208670
17. Knockdown of MADD can render cells susceptible to spontaneous apoptosis but had no discernible effect on cell proliferation, colony size or cell cycle progression. MADD alone is sufficient and necessary for cancer cell survival. PMID: 16682944
18. MADD has a role in the control of cancer cell survival/death as a negative regulator of caspase-8 and confers significant resistance to TRAIL-induced apoptosis PMID: 17314102
19. data strongly indicate that germline mutations in SPI1 and MADD genes do not confer a high risk of chronic lymphocytic leukaemia and do not make a major contribution to the familial risk of the disease PMID: 17410194
20. we have identified an essential role for splice variants of the IG20 gene in TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)resistance. PMID: 18223207
21. Expression of KIAA0358 exerted a potent antiapoptotic effect in both the SK-N-SH and SH-SY5Y neuroblastoma cell lines, whereas expression of IG20-SV4 had proapoptotic effects directly related to the activation of caspase-8 in these cells. PMID: 18794122
22. KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through MADD. PMID: 18849981
23. Loss of MADD expression resulted in reduced Grb2 and Sos1/2 recruitment to the TNFR1 complex and decreased Ras and MEKK1/2 activation PMID: 19289468
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亞細胞定位：

Cell membrane. Cytoplasm. Cell projection, axon.
蛋白家族：

MADD family
組織特異性：

Expressed in testis, ovary, brain and heart. Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon. Expressed in liver.; [Isoform 1]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and
數據庫鏈接：

HGNC: 6766

OMIM: 603584

KEGG: hsa:8567

STRING: 9606.ENSP00000310933

UniGene: Hs.82548