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  4. Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3A)

Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3A)

  • 中文名稱：
    人IDH3A重組蛋白
  • 貨號：
    CSB-YP010991HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人IDH3A重組蛋白
  • 貨號：
    CSB-EP010991HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人IDH3A重組蛋白
  • 貨號：
    CSB-EP010991HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人IDH3A重組蛋白
  • 貨號：
    CSB-BP010991HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人IDH3A重組蛋白
  • 貨號：
    CSB-MP010991HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IDH3A
  • Uniprot No.：
  • 別名：
    H IDH alpha; HIDH alpha; IDH3A; IDH3A_HUMAN; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha; mitochondrial; NAD ; NAD(+)-specific ICDH subunit alpha; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor ; NAD+ specific ICDH; NADsubunit alpha mitochondrial precursor ; OTTHUMP00000184906
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    28-366
  • 氨基酸序列
    TGG VQTVTLIPGD GIGPEISAAV MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PSEAKESMDK NKMGLKGPLK TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE GEYSGIEHVI VDGVVQSIKL ITEGASKRIA EFAFEYARNN HRSNVTAVHK ANIMRMSDGL FLQKCREVAE SCKDIKFNEM YLDTVCLNMV QDPSQFDVLV MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD MANPTALLLS AVMMLRHMGL FDHAARIEAA CFATIKDGKS LTKDLGGNAK CSDFTEEICR RVKDLD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
  • 基因功能參考文獻：
    1. The silencing of IDH3a significantly delayed tumor growth by suppressing the HIF-1-mediated Warburg effect and angiogenesis. PMID: 25531325
    2. for IDH, a normal alpha subunit is required for catalytic activity and alpha-Arg88 likely participates in the isocitrate site, whereas the beta and gamma subunits have roles in the nucleotide functions of this allosteric enzyme PMID: 14555658
    3. active sites of the human NAD-IDH are shared between alpha and gamma subunits and between alpha and beta subunits PMID: 16737955
    4. Asp181 is essential for nicotinamide-adenine dinucleotide (NAD)-specific IDH alpha subunit catalysis and may also facilitate the binding of substrates. PMID: 17432878
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Isocitrate and isopropylmalate dehydrogenases family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5384

    OMIM: 601149

    KEGG: hsa:3419

    STRING: 9606.ENSP00000299518

    UniGene: Hs.591110



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