Recombinant Human Granzyme B (GZMB)

中文名稱：

人GZMB重組蛋白
貨號：

CSB-EP010082HUa6
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

GZMB
Uniprot No.：

P10144
別名：

(C11)(CTLA-1)(Cathepsin G-like 1)(CTSGL1)(Cytotoxic T-lymphocyte proteinase 2)(Lymphocyte protease)(Fragmentin-2)(Granzyme-2)(Human lymphocyte protein)(HLP)(SECT)(T-cell serine protease 1-3E)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

39.5 kDa
表達區域：

21-247aa
氨基酸序列

IIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-B2M-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
基因功能參考文獻：
1. our exploratory study suggests that EOMES, BCL6 and GZMB gene expression are aberrant within the PB T cell transcriptome of HT patients. The association of this transcription signature with the heterogeneity of HT and disease control is suggested. PMID: 29319368
2. Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
3. Authors found that R(48)-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q(48)-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. PMID: 28653095
4. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
5. Study provides evidence that Il10 expression is limited to migratory CD25++ regulatory T cells only. PMID: 27756896
6. Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up PMID: 27230446
7. Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner. PMID: 28338658
8. Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
9. Proteolysis by granzyme B enhances presentation of autoantigenic PAD4 epitopes in rheumatoid arthritis. PMID: 27700100
10. our results suggest granzyme B PET imaging can serve as a quantitatively useful predictive biomarker for efficacious responses to cancer immunotherapy. PMID: 28461564
11. Plasma GzmB levels may reflect the degree of pruritus and dermatitis in patients with atopic dermatitis PMID: 27686401
12. These results suggest the critical importance of miR-378 in the regulation of GrzB expression and a protective role for GrzB in controlling dengue virus replication in vivo. PMID: 26166761
13. Analysis of infiltrating granzyme B-expressing T cells at the invasive borders of the colon tumors revealed a significant difference in expression granzyme by race underlining decreased antitumor cytotoxic immunity in African Americans. PMID: 27310868
14. Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed. PMID: 27846431
15. granzyme-B levels were found to be significantly associated with increased insulin resistance in adolescent polycystic ovary syndrome patients. Additionally, elevated levels of serum granzyme-B were predictive for increased cardiovascular risk in PCOS patients PMID: 26802256
16. results suggest that enhanced IL-21R expression of CD19(+)CD5(+) B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19(+)CD5(+) B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. PMID: 26884645
17. Findings show that GrB was produced in 57.1% colorectal cancer cell (CRC) lines and 100% CRC-derived Cancer Stem Cells and present a novel role for GrB as up-modulator of epitelial-to-mesenchimal transition in CRC cells. PMID: 26830472
18. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
19. Data show that NK cell lines could secreted rapidly inactive Mr 35 000 granzyme B (GZB)outside secretory lysosomes (SLs). PMID: 26927382
20. Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9. PMID: 26670609
21. these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
22. Among SLAMF4+ cells, the T cell fraction positive for perforin and granzyme B was higher in those obtained from healthy donors, while the percentage of T cells that were single-positive for granzyme B was higher in cells obtained from patients with SLE. PMID: 26314831
23. Costimulation blockade by abatacept can decrease the serum levels of GZMB in rheumatoid arthritis patients responding to the treatment. PMID: 26633185
24. elevated in the inflammatory lesions of placentas with villitis of unknown etiology PMID: 25725937
25. Combined evaluation of granzyme B and perforin may have a role in noninvasive diagnosis of acute rejection after kidney transplantation. PMID: 25643139
26. this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
27. identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
28. GB-induced ROS significantly promote apoptosis. PMID: 25361078
29. Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with granzymeB+ CD8+ T-cell infiltration. PMID: 25671296
30. Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV infected individuals. PMID: 25531694
31. GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. PMID: 26410968
32. PFN appears to form arc structures on target membranes that serve as minimally disrupting conduits for GzmB translocation. PMID: 25146929
33. These results suggest that GZMB Q55R, P94A, and Y247H polymorphisms are not significantly associated with colon cancer incidence, or metastasis to lymph node and distant organ. PMID: 25313744
34. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. PMID: 25780036
35. Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
36. The results of study found implicate possible effect of this genetic polymorphism in susceptibility to SSPE which needs to be confirmed in bigger populations. PMID: 24875585
37. Activated CD4 and CD8 T cells secrete similar amounts of GrzB. PMID: 25245659
38. The suppression-abrogating cytokine IL-6 augments GzmB expression by human CD4 T cells, and it inhibits Treg suppression via a nonapoptotic GzmB-mediated mechanism. PMID: 25637022
39. Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells. PMID: 25367116
40. CASP is specifically cleaved by granzymeB. PMID: 25159843
41. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
42. The GrB-Sb9 nexus may therefore represent an additional mechanism of limiting lymphocyte lifespan and populations. PMID: 24488096
43. our data indicate that the co-expression of perforin and granzyme B genes exhibits anticancer potential PMID: 24696715
44. Cancer cells released soluble factors that inhibited granzyme B, perforin and IFN-gamma production. PMID: 24894428
45. multiple linear regression analysis revealed that both type 2 diabetes mellitus and central obesity were predicting factors for GzmB, findings reveal a possible role of GzmB in type 2 diabetes mellitus. PMID: 24195710
46. cleaves proIL-18 into active form and promotes apoptosis in the inflammed skin PMID: 23820889
47. These results suggest that GrzB from CCR5+ memory CD4 T cells may have a role in cellular and tissue pathologies during HIV infection. PMID: 24999042
48. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage. PMID: 24835396
49. Report increased granzyme B expression in discoid lupus erythematosus. PMID: 23980805
50. Unlike IL-18 and FKN, plasma GZB may have a role in severity of acute coronary syndrome PMID: 24307760
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亞細胞定位：

Secreted. Cytolytic granule.
蛋白家族：

Peptidase S1 family, Granzyme subfamily
數據庫鏈接：

HGNC: 4709

OMIM: 123910

KEGG: hsa:3002

STRING: 9606.ENSP00000216341

UniGene: Hs.1051