在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

GZMB Recombinant Monoclonal Antibody

  • 中文名稱:
    GZMB重組抗體
  • 貨號:
    CSB-RA794900A0HU
  • 規格:
    ¥1320
  • 圖片:
    • IHC image of CSB-RA794900A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA794900A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他:

產品詳情

  • 產品描述:
    CSB-RA794900A0HU GZMB重組單克隆抗體是經ELISA、免疫組化(IHC)和免疫熒光(IF)嚴格驗證的高特異性科研工具。該抗體靶向顆粒酶B(Granzyme B),該蛋白酶在細胞毒性T淋巴細胞和自然殺傷細胞的顆粒中富集,通過激活caspase依賴性凋亡通路參與抗病毒及抗腫瘤免疫應答。通過重組表達技術制備的單克隆抗體展現出優異的批間穩定性,在石蠟包埋組織切片中可實現1:50-1:200的寬泛稀釋范圍,在細胞免疫熒光實驗中支持1:20-1:200的梯度應用,精準定位胞漿內GZMB的分布特征。實驗數據表明其能有效識別天然及重組蛋白,在腫瘤微環境分析、免疫細胞活性評估及自身免疫性疾病機制研究中具有重要應用價值,尤其適用于檢測感染模型、腫瘤浸潤淋巴細胞或炎癥病灶中效應免疫細胞的活化狀態。該產品經抗原親和純化處理,適用于福爾馬林固定樣本和冰凍切片,為免疫調控研究提供可靠的技術支持。
  • Uniprot No.:
  • 基因名:
  • 別名:
    Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E), GZMB, CGL1 CSPB CTLA1 GRB
  • 反應種屬:
    Human
  • 免疫原:
    A synthesized peptide derived from human Granzyme B
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    6A1
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, IHC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 基因功能參考文獻:
    1. our exploratory study suggests that EOMES, BCL6 and GZMB gene expression are aberrant within the PB T cell transcriptome of HT patients. The association of this transcription signature with the heterogeneity of HT and disease control is suggested. PMID: 29319368
    2. Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
    3. Authors found that R(48)-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q(48)-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. PMID: 28653095
    4. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
    5. Study provides evidence that Il10 expression is limited to migratory CD25++ regulatory T cells only. PMID: 27756896
    6. Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up PMID: 27230446
    7. Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner. PMID: 28338658
    8. Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
    9. Proteolysis by granzyme B enhances presentation of autoantigenic PAD4 epitopes in rheumatoid arthritis. PMID: 27700100
    10. our results suggest granzyme B PET imaging can serve as a quantitatively useful predictive biomarker for efficacious responses to cancer immunotherapy. PMID: 28461564
    11. Plasma GzmB levels may reflect the degree of pruritus and dermatitis in patients with atopic dermatitis PMID: 27686401
    12. These results suggest the critical importance of miR-378 in the regulation of GrzB expression and a protective role for GrzB in controlling dengue virus replication in vivo. PMID: 26166761
    13. Analysis of infiltrating granzyme B-expressing T cells at the invasive borders of the colon tumors revealed a significant difference in expression granzyme by race underlining decreased antitumor cytotoxic immunity in African Americans. PMID: 27310868
    14. Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed. PMID: 27846431
    15. granzyme-B levels were found to be significantly associated with increased insulin resistance in adolescent polycystic ovary syndrome patients. Additionally, elevated levels of serum granzyme-B were predictive for increased cardiovascular risk in PCOS patients PMID: 26802256
    16. results suggest that enhanced IL-21R expression of CD19(+)CD5(+) B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19(+)CD5(+) B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. PMID: 26884645
    17. Findings show that GrB was produced in 57.1% colorectal cancer cell (CRC) lines and 100% CRC-derived Cancer Stem Cells and present a novel role for GrB as up-modulator of epitelial-to-mesenchimal transition in CRC cells. PMID: 26830472
    18. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
    19. Data show that NK cell lines could secreted rapidly inactive Mr 35 000 granzyme B (GZB)outside secretory lysosomes (SLs). PMID: 26927382
    20. Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9. PMID: 26670609
    21. these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
    22. Among SLAMF4+ cells, the T cell fraction positive for perforin and granzyme B was higher in those obtained from healthy donors, while the percentage of T cells that were single-positive for granzyme B was higher in cells obtained from patients with SLE. PMID: 26314831
    23. Costimulation blockade by abatacept can decrease the serum levels of GZMB in rheumatoid arthritis patients responding to the treatment. PMID: 26633185
    24. elevated in the inflammatory lesions of placentas with villitis of unknown etiology PMID: 25725937
    25. Combined evaluation of granzyme B and perforin may have a role in noninvasive diagnosis of acute rejection after kidney transplantation. PMID: 25643139
    26. this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
    27. identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
    28. GB-induced ROS significantly promote apoptosis. PMID: 25361078
    29. Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with granzymeB+ CD8+ T-cell infiltration. PMID: 25671296
    30. Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV infected individuals. PMID: 25531694
    31. GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. PMID: 26410968
    32. PFN appears to form arc structures on target membranes that serve as minimally disrupting conduits for GzmB translocation. PMID: 25146929
    33. These results suggest that GZMB Q55R, P94A, and Y247H polymorphisms are not significantly associated with colon cancer incidence, or metastasis to lymph node and distant organ. PMID: 25313744
    34. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. PMID: 25780036
    35. Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
    36. The results of study found implicate possible effect of this genetic polymorphism in susceptibility to SSPE which needs to be confirmed in bigger populations. PMID: 24875585
    37. Activated CD4 and CD8 T cells secrete similar amounts of GrzB. PMID: 25245659
    38. The suppression-abrogating cytokine IL-6 augments GzmB expression by human CD4 T cells, and it inhibits Treg suppression via a nonapoptotic GzmB-mediated mechanism. PMID: 25637022
    39. Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells. PMID: 25367116
    40. CASP is specifically cleaved by granzymeB. PMID: 25159843
    41. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
    42. The GrB-Sb9 nexus may therefore represent an additional mechanism of limiting lymphocyte lifespan and populations. PMID: 24488096
    43. our data indicate that the co-expression of perforin and granzyme B genes exhibits anticancer potential PMID: 24696715
    44. Cancer cells released soluble factors that inhibited granzyme B, perforin and IFN-gamma production. PMID: 24894428
    45. multiple linear regression analysis revealed that both type 2 diabetes mellitus and central obesity were predicting factors for GzmB, findings reveal a possible role of GzmB in type 2 diabetes mellitus. PMID: 24195710
    46. cleaves proIL-18 into active form and promotes apoptosis in the inflammed skin PMID: 23820889
    47. These results suggest that GrzB from CCR5+ memory CD4 T cells may have a role in cellular and tissue pathologies during HIV infection. PMID: 24999042
    48. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage. PMID: 24835396
    49. Report increased granzyme B expression in discoid lupus erythematosus. PMID: 23980805
    50. Unlike IL-18 and FKN, plasma GZB may have a role in severity of acute coronary syndrome PMID: 24307760

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted. Cytolytic granule.
  • 蛋白家族:
    Peptidase S1 family, Granzyme subfamily
  • 數據庫鏈接:

    HGNC: 4709

    OMIM: 123910

    KEGG: hsa:3002

    STRING: 9606.ENSP00000216341

    UniGene: Hs.1051



主站蜘蛛池模板: 玩弄japan白嫩少妇hd| 日本欧美视频在线观看| 国产免费无遮挡吸乳视频| 亚洲人成小说网站色在线| 蜜臀精品国产高清在线观看| 日韩精品电影综合区亚洲| 精品成人av一区二区三区| 99久久国产宗和精品1上映| 制服视频在线一区二区| 老湿机香蕉久久久久久| 免费做a爰片久久毛片a片| 国产精品女同一区二区| 久久中文字幕乱码久久午夜| 欧美成人精品第一区| 中文字幕精品亚洲无线码一区| 欧美日韩精品一区二区视频| 国产在线高清亚洲精品二区| 久久经精品久久精品免费观看| 伊伊人成亚洲综合人网7777| 少妇内射兰兰久久| 亚洲一区爱区精品无码| 色诱视频在线观看| 人人做人人爽人人爱| 女人被狂c到高潮视频网站| 他掀开裙子把舌头伸进去添视频 | 亚洲综合另类小说色区色噜噜 | 欧美亚洲精品一区二区| 久久电影网午夜鲁丝片免费| 欧洲美熟女乱又伦免费视频| 好紧好爽好深再快点av在线| 国产精品三级一区二区| 国产欧美日韩专区发布| 一本aⅴ高清一区二区三区| 最近中文av字幕在线中文| 人人妻人人澡人人爽人人精品av | 无码任你躁久久久久久老妇| 人妻无码久久中文字幕专区| 五月综合激情婷婷六月色窝| 99久久久无码国产精品性| 免费人成在线观看成人片| 亚洲午夜福利院在线观看|