Recombinant Human General transcription and DNA repair factor IIH helicase subunit XPB (ERCC3)

^{In Stock}

中文名稱：

人ERCC3重組蛋白
貨號：

CSB-EP007771HU
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

ERCC3
Uniprot No.：

P19447
別名：

(TFIIH subunit XPB)(Basic transcription factor 2 89 kDa subunit)(BTF2 p89)(DNA excision repair protein ERCC-3)(DNA repair protein complementing XP-B cells)(TFIIH basal transcription factor complex 89 kDa subunit)(TFIIH 89 kDa subunit)(TFIIH p89)(Xeroderma pigmentosum group B-complementing protein)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

115.6 kDa
表達區域：

1-782aa
氨基酸序列

MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal GST-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
基因功能參考文獻：
1. This study demonstrates that CpG-specific DNA methylation in the ERCC3 promoter region may be involved in benzene-induced epigenetic modification and it may contribute to benzene-induced hematotoxicity. PMID: 28813025
2. An essential role of MYC-ERCC3 interactions in PDAC. PMID: 27384421
3. similar to tumors arising in the background of homologous repair defects, mutations in nucleotide excision repair genes such as ERCC3 could constitute potential therapeutic targets in a subset of hereditary breast cancers. PMID: 27655433
4. results reveal a previously unknown role for transcription factor IIH in ATR kinase activation in non-replicating, non-cycling cells PMID: 28592488
5. Study shows that: mRNA synthesis is sensitive to the inhibition of the ATPase activity of XPB; mRNA synthesis accommodates the depletion of XPB; XPB-depleted TFIIH participates in mRNA synthesis, and finally, XPB ATPase overcomes transcription initiation block imposed by its helicase motifs. PMID: 28157507
6. findings suggest that benzene exposure may be associated with hypermethylation in ERCC3, and that genetic variants in EPHX1 may play an important role in epigenetic changes and hematotoxicity among benzene-exposed workers PMID: 23797950
7. The crystal structure of the C-terminal half of the XPB subunit of TFIIH (residues 494-782) is reported, containing XPB helicase domain (HD)2 and a C-terminal extension which shares structural similarity with RIG-I. PMID: 23385459
8. Phenotype-specific adverse effects of XPD mutations on human prenatal development implicate impairment of TFIIH-mediated functions in placenta. PMID: 22234153
9. reduction in ERCC3 by siRNA interference in human melanocytes in vitro reduced their tyrosinase production ability PMID: 22615732
10. These results indicated that persistent HBV infection might trigger NER impairment in part through upregulation of miR-192, which suppressed the levels of ERCC3 and ERCC4. PMID: 21672525
11. Results show that a deficiency in functional XPB paradoxically renders cells more sensitive to the genotoxic effects of oxidative stress while reducing the cytotoxic effects. PMID: 19840190
12. The phosphorylation of the androgen receptor by TFIIH directs the ubiquitin/proteasome process. PMID: 21157430
13. The C1D gene has been identified as one of the major genes whose expression is significantly upregulated by restoring XPB function. PMID: 20530579
14. results demonstrated that a) XP-G/CS mutations affect the disassembly state of TFIIH resulting in the dissociation of CAK, but not XPD from core TFIIH PMID: 20543986
15. TFIIH core subunit p89, but not other subunits of TFIIH, associates with the centrosomes and the adjacent parts of the mitotic spindle during mitosis. PMID: 20208140
16. p52 mediates function within the transcription/repair factor TFIIH. PMID: 12080057
17. Reduced level of the repair/transcription factor TFIIH in trichothiodystrophy. PMID: 12393803
18. TFIIH from trichothiodystrophy patients, but not from xeroderma pigmentosum patients, exhibit a significant in vitro basal transcription defect in addition to a reduced intercellular concentration. PMID: 12820975
19. High XRB mRNA levels is associated with Clear cell tumors of epithelial ovarian cancer PMID: 14614013
20. identification of a new, evolutionarily conserved, core TFIIH subunit is essential for our understanding of TFIIH function in transcription, DNA repair and human disease PMID: 15220921
21. Reduced expression of ERCC3 was correlated with esophageal cancer progression PMID: 15375507
22. data support an additional role for XPB in promoting the incision of the damaged DNA fragment and reveal a point of nucleotide excision repair regulation on TFIIH without interference in its transcription activity PMID: 15549133
23. Single Nucleotide Polymorphism in the ERCC3 is associated with lung cancer PMID: 16835333
24. XPB could inhibit the proliferation of hepatoma cells and had a positive effect on the expression of p53 and p21(waf1/cip1) but a negative effect on c-myc. PMID: 16914395
25. Multiple proteins both known and unknown to interact with RAD52 were identified by the "dual-tagging" proteomic method. PMID: 19338310
26. Single Nucleotide Polimorphisms in VEGF and ERCC3 were associated with alterations in White Blood Cells and WBC subtype counts in workers exposed to benzene, even at relatively low levels of exposure below 1 ppm. PMID: 19773279
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相關疾病：

Xeroderma pigmentosum complementation group B (XP-B); Trichothiodystrophy 2, photosensitive (TTD2)
亞細胞定位：

Nucleus.
蛋白家族：

Helicase family, RAD25/XPB subfamily
數據庫鏈接：

HGNC: 3435

OMIM: 133510

KEGG: hsa:2071

STRING: 9606.ENSP00000285398

UniGene: Hs.469872