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Recombinant Human DNA nucleotidylexotransferase (DNTT)

  • 中文名稱:
    人DNTT重組蛋白
  • 貨號:
    CSB-YP007088HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人DNTT重組蛋白
  • 貨號:
    CSB-EP007088HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人DNTT重組蛋白
  • 貨號:
    CSB-BP007088HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人DNTT重組蛋白
  • 貨號:
    CSB-MP007088HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; TDT; TDT_HUMAN; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase; Terminal transferase
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-509
  • 氨基酸序列
    MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKG FRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMT GKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCE FRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAV LNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLV SCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQL LQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSS WQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKT KRIFLKAESEEEIFAHLGLDYIEPWERNA
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
  • 基因功能參考文獻:
    1. TdT protein can be found in cells of epithelial origin and specifically sebaceous cells, both benign and malignant. PMID: 28677299
    2. Structural basis for a new templated activity by terminal deoxynucleotidyl transferase and implications for V(D)J recombination have been described. PMID: 27499438
    3. TdT-positive cells may be part of the inflammatory milieu in infant kidneys. PMID: 28248816
    4. Absence of TdT expression identifies a subset of high-risk T-acute lymphoblastic leukemia/lymphoma that overlaps with, but is not identical to, the ETP leukemia, providing additional prognostic value. PMID: 23702731
    5. Our study confirms that PAX5 and TdT expression can be expressed in a high percentage of Merkel cell carcinomas and so when positive are not diagnostic of lymphoblastic leukemia/lymphoma. PMID: 23329999
    6. role in DNA repair PMID: 11974916
    7. Overexpression of newly discovered alternatively spliced short or long human TdT isoforms greatly reduces the efficiency of recombination, which is reverted to normal levels by the simultaneous expression of both enzymes. PMID: 15356150
    8. In spleen, appendix and branchial cleft cysts the range of TdT-positivity was 0-13, 0-96 and 0-6 TdT+ cells per high-power field. PMID: 16885057
    9. The TdT binding, DNA binding and dimerization regions, and nuclear localization signal (NLS) in TdIF1, were identified. PMID: 17663723

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  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    DNA polymerase type-X family
  • 數據庫鏈接:

    HGNC: 2983

    OMIM: 187410

    KEGG: hsa:1791

    STRING: 9606.ENSP00000360216

    UniGene: Hs.534206



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