在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Human DNA ligase 1 (LIG1)

  • 中文名稱(chēng):
    Recombinant Human DNA ligase 1 (LIG1)
  • 品名簡(jiǎn)稱(chēng):
    Recombinant Human LIG1 protein
  • 貨號(hào):
    CSB-EP012930HUf0
  • 說(shuō)明書(shū):
  • 規(guī)格:
    ¥1344
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
  • Uniprot No.:
  • 別名:
    DNA ligase I;Polydeoxyribonucleotide synthase [ATP] 1
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度:
    Full Length
  • 來(lái)源:
    E.coli
  • 分子量:
    133.3 kDa
  • 表達(dá)區(qū)域:
    1-919aa
  • 氨基酸序列
    MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    C-terminal GST-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • 基因功能參考文獻(xiàn):
    1. single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase PMID: 28751376
    2. A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID: 28803780
    3. The rs156641 polymorphism of DNA ligase 1 (LIG1) was significantly associated with lung cancer risk, whereas no association was found between rs3730931/rs439132/rs20579 polymorphisms and lung cancer. PMID: 27352326
    4. Data suggest that DNA ligase I (LigI)-deficient 46BR.1G1 cells represent a model to investigate the biological effects of sub-lethal levels of DNA insults. PMID: 26151554
    5. The LIG1 CC genotype was associated with susceptibility to non-small cell lung cancer, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes. PMID: 26125914
    6. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
    7. Ppolymorphisms in LIG1 affect its expression and may therefore change its function. PMID: 25189241
    8. there is no association between LIGI polymorphisms and cervical cancer risk. However, they may be playing an important role in modulating the risk of cervical adenocarcinoma in North Indian women PMID: 24084463
    9. DNA ligase I also interacts with replication factor C, the factor that loads the PCNA trimeric ring onto DNA. PMID: 22918593
    10. Data indicate that Ku70/Ku80 facilitates the cooperative binding of multiple XRCC4/Ligase IV (XL) and XLF molecules to DNA. PMID: 23620595
    11. Single nucleotide polymorphisms in LIG1 are associated with myelodysplastic syndromes. PMID: 23339595
    12. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
    13. Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK. PMID: 22139380
    14. Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency. PMID: 21561855
    15. RNA silencing of human DNA ligase I expression severely reduced replication of viral DNA in cells infected with vaccinia virus ligase-deficient mutants. PMID: 20006844
    16. DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro. PMID: 11948189
    17. stimulated by APE1 for progression through the base excision repair pathway PMID: 12200445
    18. phosphorylation of DNA ligase I and possibly other replicative enzymes is part of the mechanism that directs the disassembly of the replication machinery at the completion of S-phase. PMID: 12851383
    19. majority of single-strand DNA interruptions produced during the repair of alkylated DNA bases are repaired by the pathway mediated by Pol beta and either Lig I or Lig III PMID: 14627836
    20. crystal structure of human DNA ligase I (residues 233 to 919) in complex with a nicked, 5' adenylated DNA intermediate PMID: 15565146
    21. The human checkpoint sensor and alternative clamp Rad9-Rad1-Hus1 can interact with and specifically stimulate DNA ligase I PMID: 15871698
    22. LIG1-deficiency reduces recombinational repair of DNA double-strand beaks. PMID: 15907772
    23. human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
    24. siRNA mediated down-regulation of DNA ligase I in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
    25. The LIG1 SNP 5'UTR showed a significant association with glioma risk. PMID: 19124499
    26. drastically reduced replicative LigI activity in 46BR.1G1 cells results in the accumulation of both single-stranded and double-stranded DNA breaks. PMID: 19223467
    27. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468
    28. The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
    29. Data support the notion that DNA ligase I participates in homology dependent pathways that deal with replication-associated lesions generated when replication fork encounters DNA damage. PMID: 19597347
    30. Data show that disruption of LigI and PCNA interactions influences trinucleotide repeat instability. PMID: 19628465

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Nucleus.
  • 蛋白家族:
    ATP-dependent DNA ligase family
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 6598

    OMIM: 126391

    KEGG: hsa:3978

    STRING: 9606.ENSP00000263274

    UniGene: Hs.1770



主站蜘蛛池模板: 成人爱做日本视频免费| 男女一边摸一边做爽爽| 成人区人妻精品一区二区不卡视频| av天堂永久资源网亚洲高清 | 毛片大全真人在线| 国产精品99久久久久久宅男| 女人大荫蒂毛茸茸视频| 免费人成网站在线观看不卡 | 亚洲人成色77777在线观看大战| 亲嘴扒胸摸屁股激烈网站| аⅴ天堂中文在线网官网| 欧美日韩国产亚洲沙发| 国产在线精品一品二区| 99久久精品费精品国产| 一本色道久久综合亚洲精品| 中国熟妇牲交视频| 亚洲欧美综合精品成人导航 | 国产午夜成人久久无码一区二区| 国产真实夫妇交换视频| 亚洲一区二区三区波多野结衣| 曰批全过程免费视频在线观看无码| 国色天香中文字幕在线视频| 自慰系列无码专区| 欧美综合精品久久久久成人影院| 亚洲国产无套无码av电影| 极品尤物一区二区三区| 在线看片免费人成视频福利| 人人澡人人澡人人看添av| 国产精品99久久99久久久动漫| 国产精品久免费的黄网站| 久久香蕉国产线看观看亚洲小说| 久久这里有精品国产电影网| 国产成人精品久久亚洲高清不卡| 亚洲精品久久午夜无码一区二区| 男女性爽大片视频免费看| 中国娇小与黑人巨大交| 少妇做爰免费视看片| 久久天天躁狠狠躁夜夜夜| 一本久久精品一区二区| 欧美丰满熟妇xxxxx| 午夜国人精品av免费看|