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LIG1 Recombinant Monoclonal Antibody

  • 中文名稱：
    LIG1重組抗體
  • 貨號(hào)：
    CSB-RA877322A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
      All lanes: DNA Ligase I antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 102, 89, 99 kDa
      Observed band size: 140 kDa
    • IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA877322A0HU LIG1 Recombinant Monoclonal Antibody 是針對(duì)DNA連接酶1（LIG1）開(kāi)發(fā)的高特異性科研用抗體。LIG1是DNA復(fù)制與修復(fù)的關(guān)鍵酶，負(fù)責(zé)催化DNA鏈末端的連接反應(yīng)，在維持基因組穩(wěn)定性和細(xì)胞周期調(diào)控中發(fā)揮重要作用。該抗體通過(guò)重組表達(dá)技術(shù)制備，具有高批次一致性和低交叉反應(yīng)性，經(jīng)嚴(yán)格驗(yàn)證適用于ELISA、Western Blot（WB）及免疫組化（IHC）等多種實(shí)驗(yàn)場(chǎng)景。在WB實(shí)驗(yàn)中，抗體可識(shí)別天然LIG1蛋白，推薦使用稀釋比例為1:500-1:5000，能清晰檢測(cè)目標(biāo)蛋白表達(dá)；IHC實(shí)驗(yàn)推薦稀釋比例為1:50-1:200，可在組織樣本中準(zhǔn)確定位LIG1的亞細(xì)胞分布。其卓越的性能使其成為研究DNA損傷修復(fù)機(jī)制、腫瘤發(fā)生發(fā)展相關(guān)信號(hào)通路及細(xì)胞周期調(diào)控的理想工具，可廣泛應(yīng)用于分子生物學(xué)、癌癥研究及遺傳穩(wěn)定性等基礎(chǔ)科研領(lǐng)域，為解析LIG1在疾病模型中的功能提供可靠支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Polydeoxyribonucleotide synthase [ATP] 1), LIG1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human DNA Ligase I
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    7B2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • 基因功能參考文獻(xiàn)：
    1. single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase PMID: 28751376
    2. A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID: 28803780
    3. The rs156641 polymorphism of DNA ligase 1 (LIG1) was significantly associated with lung cancer risk, whereas no association was found between rs3730931/rs439132/rs20579 polymorphisms and lung cancer. PMID: 27352326
    4. Data suggest that DNA ligase I (LigI)-deficient 46BR.1G1 cells represent a model to investigate the biological effects of sub-lethal levels of DNA insults. PMID: 26151554
    5. The LIG1 CC genotype was associated with susceptibility to non-small cell lung cancer, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes. PMID: 26125914
    6. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
    7. Ppolymorphisms in LIG1 affect its expression and may therefore change its function. PMID: 25189241
    8. there is no association between LIGI polymorphisms and cervical cancer risk. However, they may be playing an important role in modulating the risk of cervical adenocarcinoma in North Indian women PMID: 24084463
    9. DNA ligase I also interacts with replication factor C, the factor that loads the PCNA trimeric ring onto DNA. PMID: 22918593
    10. Data indicate that Ku70/Ku80 facilitates the cooperative binding of multiple XRCC4/Ligase IV (XL) and XLF molecules to DNA. PMID: 23620595
    11. Single nucleotide polymorphisms in LIG1 are associated with myelodysplastic syndromes. PMID: 23339595
    12. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
    13. Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK. PMID: 22139380
    14. Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency. PMID: 21561855
    15. RNA silencing of human DNA ligase I expression severely reduced replication of viral DNA in cells infected with vaccinia virus ligase-deficient mutants. PMID: 20006844
    16. DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro. PMID: 11948189
    17. stimulated by APE1 for progression through the base excision repair pathway PMID: 12200445
    18. phosphorylation of DNA ligase I and possibly other replicative enzymes is part of the mechanism that directs the disassembly of the replication machinery at the completion of S-phase. PMID: 12851383
    19. majority of single-strand DNA interruptions produced during the repair of alkylated DNA bases are repaired by the pathway mediated by Pol beta and either Lig I or Lig III PMID: 14627836
    20. crystal structure of human DNA ligase I (residues 233 to 919) in complex with a nicked, 5' adenylated DNA intermediate PMID: 15565146
    21. The human checkpoint sensor and alternative clamp Rad9-Rad1-Hus1 can interact with and specifically stimulate DNA ligase I PMID: 15871698
    22. LIG1-deficiency reduces recombinational repair of DNA double-strand beaks. PMID: 15907772
    23. human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
    24. siRNA mediated down-regulation of DNA ligase I in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
    25. The LIG1 SNP 5'UTR showed a significant association with glioma risk. PMID: 19124499
    26. drastically reduced replicative LigI activity in 46BR.1G1 cells results in the accumulation of both single-stranded and double-stranded DNA breaks. PMID: 19223467
    27. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468
    28. The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
    29. Data support the notion that DNA ligase I participates in homology dependent pathways that deal with replication-associated lesions generated when replication fork encounters DNA damage. PMID: 19597347
    30. Data show that disruption of LigI and PCNA interactions influences trinucleotide repeat instability. PMID: 19628465

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    ATP-dependent DNA ligase family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 6598

    OMIM: 126391

    KEGG: hsa:3978

    STRING: 9606.ENSP00000263274

    UniGene: Hs.1770



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