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Recombinant Human DNA-directed RNA polymerase II subunit RPB2 (POLR2B), partial

  • 中文名稱:
    人POLR2B重組蛋白
  • 貨號:
    CSB-YP018328HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人POLR2B重組蛋白
  • 貨號:
    CSB-EP018328HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    人POLR2B重組蛋白
  • 貨號:
    CSB-EP018328HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人POLR2B重組蛋白
  • 貨號:
    CSB-BP018328HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人POLR2B重組蛋白
  • 貨號:
    CSB-MP018328HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    POLR2B
  • Uniprot No.:
  • 別名:
    POLR2B; DNA-directed RNA polymerase II subunit RPB2; EC 2.7.7.6; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
  • 基因功能參考文獻:
    1. Studies indicate transcription of many genes, is regulated after the initiation step, by pausing of RNA polymerase II during elongation through the promoter-proximal region. PMID: 21081187
    2. Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation PMID: 12050112
    3. The highly conserved glutamic acid 791 of Rpb2 is involved in the binding of magnesium and is required for both for the polymerization and cleavage reactions. PMID: 15886393
    4. the human Maf1 protein negatively regulates transcription by all three nuclear Pols, Pol I, Pol II and Pol III PMID: 17499043
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    RNA polymerase beta chain family
  • 數據庫鏈接:

    HGNC: 9188

    OMIM: 180661

    KEGG: hsa:5431

    STRING: 9606.ENSP00000312735

    UniGene: Hs.602757



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