在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁(yè)
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM)

Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM)

  • 中文名稱：
    人POLM重組蛋白
  • 貨號(hào)：
    CSB-MP889065HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：
  • 中文名稱：
    人POLM重組蛋白
  • 貨號(hào)：
    CSB-EP889065HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Name:POLM Synonyms:polmu
  • Uniprot No.：
  • 別名：
    DNA-directed DNA polymerase mu; DNA-directed DNA/RNA polymerase mu; DPOLM_HUMAN; FLJ35482; Pol iota; Pol Mu; Polm; POLM protein; Polymerase (DNA directed) mu; Polymerase DNA directed mu; Tdt N; Tdt-N; TdtN; Terminal transferase
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-494
  • 氨基酸序列
    MLPKRRRARV GSPSGDAASS TPPSTRFPGV AIYLVEPRMG RSRRAFLTGL ARSKGFRVLD ACSSEATHVV MEETSAEEAV SWQERRMAAA PPGCTPPALL DISWLTESLG AGQPVPVECR HRLEVAGPRK GPLSPAWMPA YACQRPTPLT HHNTGLSEAL EILAEAAGFE GSEGRLLTFC RAASVLKALP SPVTTLSQLQ GLPHFGEHSS RVVQELLEHG VCEEVERVRR SERYQTMKLF TQIFGVGVKT ADRWYREGLR TLDDLREQPQ KLTQQQKAGL QHHQDLSTPV LRSDVDALQQ VVEEAVGQAL PGATVTLTGG FRRGKLQGHD VDFLITHPKE GQEAGLLPRV MCRLQDQGLI LYHQHQHSCC ESPTRLAQQS HMDAFERSFC IFRLPQPPGA AVGGSTRPCP SWKAVRVDLV VAPVSQFPFA LLGWTGSKLF QRELRRFSRK EKGLWLNSHG LFDPEQKTFF QAASEEDIFR HLGLEYLPPE QRNA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
  • 基因功能參考文獻(xiàn)：
    1. Polmu point mutations affecting 2 conserved adjacent residues located in the 8 kDa domain, G174S and R175H, limit the efficiency of accurate NHEJ by Polmu in vitro and in vivo due to decreased template dependency during NHEJ, which renders the error-rate of the mutants higher due to the ability of Polmu to randomly incorporate nucleotides at DSBs. PMID: 28973441
    2. Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu has been described. PMID: 28911097
    3. analysis of template-dependent synthesis by human polymerase mu PMID: 26240373
    4. A study of how Polmu fixes and/or orients the mobile Loop1 part of the protein in accordance with the substrate on which it is polymerizing. PMID: 24878922
    5. specific loop 1 residues contribute to Pol mu's unique ability to catalyze template-dependent NHEJ of DSBs with unpaired 3' ends PMID: 24487959
    6. evidence suggests that Polmu could be regulated in vivo by phosphorylation of the BRCT domain (Ser12/Thr21) and of Ser372, affecting the function of loop1; Polmu's most distinctive activities would be turned off at specific cell-cycle phases (S and G2), when these functions might be harmful to the cell PMID: 23933132
    7. A specific N-terminal extension of the 8 kDa domain of DNA polymerase mu is potentially implicated in the maintenance of a closed conformation throughout the catalytic cycle, and this study indicated that it could be a target of Cdk phosphorylation. PMID: 23935073
    8. A physiological concentration of Mn(2+) ions did benefit Polmicro-mediated non-homologous end joining by improving the efficiency and accuracy of nucleotide insertion. PMID: 23275568
    9. The study points at human Polmicro residues His(329) and Arg(387) as responsible for regulating nucleotide expansions occurring during DNA repair transactions, either promoting or blocking, respectively, iterative polymerization. PMID: 23143108
    10. The results uncovered a new DNA-binding function for the BRCT domain of Polmicro and demonstrated the importance of several residues located at the primer-binding region, for both DNA-binding and polymerization activities. PMID: 23034807
    11. Pol mu binds to DNA through its amino-terminal and pol beta-like regions. PMID: 22897684
    12. DNA polymerase mu performs DNA synthesis at a AAF lesion PMID: 11972346
    13. Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. PMID: 12077346
    14. DNA polymerase mu acts in response to several types of DNA damage with a lesion bypass mechanism PMID: 12228225
    15. expression in B-cell non-Hodgkin's lymphomas PMID: 12368208
    16. Pol mu's substrate specificity is similar to that of pol beta in most respects but has an approximately 1,000-fold-reduced ability to discriminate against ribonucleotides compared to pol beta PMID: 12640116
    17. human DNA polymerase mu has a template-dependent, sequence-independent nucleotidyl transferase activity PMID: 14581466
    18. Poliota incorporates a C opposite the gamma-HOPdG adduct with nearly the same efficiency as opposite a nonadducted G residue. The subsequent extension step is performed by Polkappa, which efficiently extends from the C incorporated opposite the adduct. PMID: 15199127
    19. DNA polymerase mu has been overexpressed, purified, and its fidelity estimated for incorporation of both deoxynucleotides and ribonucleotides based on pre-steady-state kinetic data under single-turnover conditions. PMID: 15504045
    20. Overexpression of DNA polymerase mu in a Burkitt's lymphoma cell line induced an increase in somatic mutations specifically targeted to G/C residues in immunoglobulin variable genes. PMID: 15520469
    21. Pol mu promotes accuracy during Ig kappa recombination. PMID: 16061182
    22. When the terminal deoxynucleotidyl transferase (TdT) loop1 was deleted, human Polmu lacked TdT activity but improved DNA-binding and DNA template-dependent polymerization. PMID: 16963491
    23. Studies shed light on the mechanism by which a rate-limited terminal transferase activity in Polmu could regulate the balance between accuracy and necessary efficiency, providing some variability during NHEJ. PMID: 19805281

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-X family
  • 組織特異性：
    Expressed in a number of tissues. Abundant in thymus.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9185

    OMIM: 606344

    KEGG: hsa:27434

    STRING: 9606.ENSP00000242248

    UniGene: Hs.596982



主站蜘蛛池模板： 无码国产玉足脚交极品网站| 人妻激情另类乱人伦人妻| 中文在线а√在线| 好吊妞视频这里有精品| 久久久久久av无码免费网站下载| 麻豆国产va免费精品高清在线| 丝袜老师高潮呻吟高潮| 亚洲精品无码不卡av| 巨乳美乳一区二区三区| 亚洲精品久久久久玩吗| 麻豆高清免费国产一区| 国产麻豆精品一区| 午夜精品久久久久久久久| 欧美激情猛片xxxⅹ大3| 成人无码视频在线观看网址| 国产爆乳成av人在线播放| 熟妇人妻一区二区三区四区| 熟女丰满老熟女熟妇| 日韩精品无码人妻一区二区三区| 无码国产精成人午夜视频| 国产精品毛片大码女人| 国产h视频在线观看| 99在线精品一区二区三区| 欧美性video高清精品| 亚洲综合在线一区二区三区| 久久精品中文字幕| 免费国产h视频在线观看| 亚洲男人的天堂www| 99久久人妻无码精品系列蜜桃 | 韩国理伦片一区二区三区在线播放| 亚洲日韩欧美一区二区三区在线| 国产少妇高潮在线观看| 国产亚洲精品久久久久丝瓜| 国产精品久久无码一区二区三区网| 亚洲成a∨人片在无码2023| 午夜精品影视国产一区在线麻豆| 1000部啪啪未满十八勿入| 毛茸茸的中国女bbw| 超薄丝袜足j好爽在线观看| 国产精品扒开腿做爽爽爽a片唱戏 久久99国产精品久久99 | 丁香婷婷激情综合俺也去|