在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Human Blood group Rh (D) polypeptide (RHD), partial

In Stock
  • 中文名稱:
    人RHD重組蛋白
  • 貨號:
    CSB-EP019677HU
  • 規格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    RHD; Blood group Rh(D polypeptide; RHXIII; Rh polypeptide 2; RhPII; Rhesus D antigen; CD antigen CD240D
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    33.6 kDa
  • 表達區域:
    388-417aa
  • 氨基酸序列
    LNLKIWKAPHEAKYFDDQVFWKFPHLAVGF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-GST-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Tris-based buffer,50% glycerol
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The N-terminal 6xHis-GST tag was fused to the gene fragment corresponding to the 388-417aa of the human RHD protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant human RHD protein. Its purity is higher than 85%. It showed an apparent molecular weight of about 34 kDa under SDS-PAGE condition.This recombinant human RHD protein may be used in cardiovascular research.

    RHD is a gene encoding a protein named blood group Rh(D) polypeptide and belongs Rh subfamily which consist of RHD, RHCE, RhAG, RhBG, and RhCG. RHD has sequence similarity to others and is restricted to tissues or cell lines expressing erythroid characters. It was proposed that the erythrocyte Rh complex is a heterotrimer of RhAG, RhD, and RhCE protein subunits. Diseases associated with RHD include Hemolytic Disease Of Fetus And Newborn, Rh-Induced and Blood Group, Rh System.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
  • 基因功能參考文獻:
    1. 4 novel RHD alleles, each characterized by a single nucleotide substitution were identified. RHD*67T, RHD*173T, and RHD*579C give rise to a weak D phenotypical expression. Their corresponding amino acid changes are predicted to be located in the membrane-spanning or intracellular domains of the RhD protein. RHD*482G is the 4th substitution. PMID: 29052223
    2. Extensive studies show that the RHD*1227A is the most prevalent DEL allele in East Asian populations and may have confounded the initial molecular studies. PMID: 29214630
    3. The most prevalent DEL allele was RHD*DEL1 (c.1227G>A), which is proven to be immunogenic. A high frequency of RHD*Psi was detected in the donors with nondeleted RHD alleles (31%), far superior to the frequency of RHD variant alleles (15.5%). PMID: 29193119
    4. Absence of the whole RHD gene is common among RhD negative blood donors from Qingdao region, and there are rich genetic polymorphisms for this locus PMID: 29188626
    5. The RHD 1227G>A mutation contributes to the molecular basis of Del phenotype in the Taiwanese population. The point mutation results in aberrant frame shift or exon deletion transcripts and generates D protein with weak antigen presenting function. PMID: 26774048
    6. In this mixed Brazilian population, the most frequent weak D types were 1, 4, 3 and 2 (frequencies of 4.35%, 2.32%, 1.46% and 0.29%, respectively; total of 8.41%) and partial D was found in 2.90% of samples carrying the RHD gene. For samples with inconclusive RhD typing, 53.33% of them presented weak and partial RHD, and 43.75% had concomitantly more than one RHD variant PMID: 27184292
    7. sequence comparisons revealed high sequence similarity between Patr_RHbeta and Hosa_RHCE, while the chimpanzee Rh gene closest to Hosa_RHD was not Patr_RHa but rather Patr_RHy PMID: 26872772
    8. Six weak D types in the Russian Federation: the most common type 3 (49.2%) and type 1 (28.6%), type 2 (14.3), type 15 (4.8%), type 4.2 (DAR) (1.6%) and type 6 (1.6%). PMID: 27459619
    9. The frequency of RhD negative homozygosity in the Cypriot population was estimated to be 7.2%, while the frequencies of RHD hemizygosity and RhD positive homozygosity was calculated to be 39.2 and 53.6%, respectively. PMID: 27036548
    10. Occurrence of partial RhD alleles in the Tunisian population. PMID: 26482434
    11. Reduced expression of D antigen is caused not only by missense mutation of the RHD gene, but also by silent mutation that may affect splicing. PMID: 26340140
    12. Loss of heterozygosity of RhD gene on chromosome 1p in acute myeloid leukemia. PMID: 25495174
    13. The data indicate that partial DEL women appear at risk of alloimmunization to the D antigen. PMID: 26033335
    14. Weak D type 4.0 appears to be the most prevalent weak D in our population. However, all samples must be sequenced in order to determine the exact subtype of weak D type 4, since weak D type 4.2 has considerable clinical importance PMID: 25369614
    15. Paternal RHD zygosity determination in Tunisians: evaluation of three molecular tests. PMID: 24960665
    16. Serologic findings of RhD alleles in Egyptians and their clinical implications. PMID: 25219636
    17. Despite the enormous diversity of RHD alleles, first-line weak D genotyping was remarkably informative, allowing for rapid classification of most samples with conspicuous RhD phenotype in Flanders, Belgium. PMID: 25413499
    18. Splicing is altered in RHD*weak D Type 2 allele, a rare variant most commonly found in Caucasians; RHD including the full-length Exon 9 is transcribed in the presence of the c.1227G>A substitution frequently carried by Asians with DEL phenotype. PMID: 25808592
    19. Among all donors 89.00% and 10.86% were D-positive and D-negative, respectively, while 0.14% (n=55) of the donors were found to be weak D-positive. PMID: 24960662
    20. The frequency of D variants detected by IAT allele RHD(M295I) was 1:272 in D negative donors. Obviously, DEL phenotype is more common in some parts of European population than initially thought. PMID: 24556127
    21. New RHD variant alleles. PMID: 25179760
    22. Currently, it seems to be difficult to observe any new RHD alleles in the Han Chinese population. D prediction in this population is easier because popular alleles are dominant, accounting for about 99.80% of alleles in D-negative people. PMID: 24333088
    23. In Han Chinese people with weak D serotyping, 8 weak D and 4 partial D alleles were found. 3 new weak D alleles (RHD weak D 95A, 779G, and 670G) and one new partial D allele (RHD130-132 del TCT) were identified. PMID: 25070883
    24. DEL/weak D-associated RHD alleles were found in 2.17% of Australian D-, C+ and/or E+ blood donors. PMID: 24894016
    25. RHD alleles and D antigen density among serologically D- C+ Brazilian blood donors. PMID: 24267268
    26. In this study, D antigen density on the erythrocyte surface of DEL individuals carrying the RHD1227A allele was extremely low, there being only very few antigenic molecules per cell, but the D antigen epitopes were grossly complete. PMID: 24333082
    27. The prevalence of D-/RHD+ samples is higher than that observed in Europeans. More than 50% of the RHD alleles found were represented by RHDpsi and RHD-CE-D(s) showing the African contribution to the genetic pool of the admixed population analyzed. PMID: 24819281
    28. A method of genotyping has been developed in the laboratory. genotyping results of 200 pregnant women have been compared with RH1 phenotype at birth. PMID: 24559796
    29. We conclude that noninvasive fetal RHD genotyping from maternal blood provides accurate results and suggests its viability as a clinical tool for the management of RhD-negative pregnant women in an admixed population. PMID: 24615044
    30. Two molecular polymorphisms to detect the (C)ce(s) type 1 haplotype. PMID: 24333080
    31. This study analyzes the phenotype and frequency of RhD and tetanus toxoid specific memory B cells in limiting dilution culture. PMID: 24965774
    32. Data indicate that non-invasive prenatal testing of cell-free fetal DNA (cffDNA) in maternal plasma can predict the fetal RhD type in D negative pregnant women. PMID: 24204719
    33. DIV alleles arose from at least two independent evolutionary events. DIV Type 1.0 with DIVa phenotype belongs to the oldest extant human RHD alleles. DIV Type 2 to Type 5 with DIVb phenotype arose from more recent gene conversions. PMID: 23461862
    34. RHD*DARA and RHD*DAR2 are the same allele. Furthermore, the alleles RHD*DAR1.2 and RHD*DAR1.3 both exist; however, the silent mutation 957G>A (V319) showed no influence on the RhD phenotype. PMID: 23902153
    35. All novel weak D types expressed all tested D epitopes. PMID: 23550956
    36. Only 0.2% of D- Polish donors carry some fragments of the RHD gene; all of them were C or E+. Almost 60% of the detected RHD alleles may be potentially immunogenic when transfused to a D- recipient. PMID: 23634715
    37. This study is the first to describe weak D types caused by intronic variations near the splice sites in the RHD gene, which is supported by the genotyping results combined with serologic profiles and bioinformatics analysis. PMID: 23216299
    38. RHD variants were identified in 91.6% of the 430 studied samples. Two of the nine previously undescribed variants, c.335G>T and c.939G>A, were found to cause aberrant mRNA splicing by means of a splicing minigene assay. PMID: 23228153
    39. Hemizygous RHD subjects demonstrated significantly higher platelet increases and peak platelet counts than homozygous RHD subjects. PMID: 23712954
    40. The RHD*weak 4.3 allele with markedly reduced antigen D expression was shown to be associated with an altered RHCE gene formation leading to the expression of C(X) and VS. PMID: 22288371
    41. modulates the influence not only of latent toxoplasmosis, but also of at least two other potentially detrimental factors, age and smoking, on human behavior and physiology. PMID: 23209579
    42. RHD*DIVa and RHCE*ceTI almost always, but not invariably, travel together. This haplotype is found in people of African ancestry and the red blood cells can demonstrate aberrant reactivity with anti-C. PMID: 22804620
    43. RHD*DOL2, like RHD*DOL1, encodes a partial D antigen and the low-prevalence antigen DAK. PMID: 22738288
    44. The use of cell-free fetal DNA in prenatal noninvasive early detection of fetal RhD status and gender by real-time PCR is highly sensitive and accurate as early as the 11th week of gestation for RhD status and the 7th week of gestation for fetal sex. PMID: 21488716
    45. This deletion appears to represent not only the first large deletion associated with weak D but also the weakest of weak D alleles so far reported. PMID: 22420867
    46. Characterization of novel RHD alleles. PMID: 22320258
    47. The RHD genotyping proved to be a necessary tool to characterise RHD alleles in donors phenotyped as D- or weak D to increase the transfusion safety in highly racial mixed population. PMID: 22211984
    48. RHD homozygotes had nearly twice as many D antigen sites as hemizygotes. Expression of c or E antigens was associated with increased RBC D antigen expression, but presence of C or e antigens reduced expression. PMID: 22121029
    49. Anti-D investigations in individuals expressing weak D Type 1 or weak D Type 2 PMID: 21658048
    50. Distribution of weak D types in the Croatian population. PMID: 21269342

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Ammonium transporter (TC 2.A.49) family, Rh subfamily
  • 組織特異性:
    Restricted to tissues or cell lines expressing erythroid characters.
  • 數據庫鏈接:

    HGNC: 10009

    OMIM: 111680

    KEGG: hsa:6007

    STRING: 9606.ENSP00000331871

    UniGene: Hs.449968



主站蜘蛛池模板: 国产欧美亚洲精品a| 四虎影视88aa久久人妻| 又大又粗又爽又黄的少妇毛片| 蜜柚av久久久久久久| 啪啪无码人妻丰满熟妇| 午夜欧美精品久久久久久久 | 国产精品人人妻人人爽人人牛| 婷婷国产成人精品视频| 国产成人啪精品视频网站| 欧美日韩亚洲国产综合乱| 日韩精品成人一区二区三区 | 日韩综合无码一区二区| 久久精品免费国产大片| 国产亚洲精品自在久久蜜tv | 久久精品2021国产| 无码av片在线观看免费| 国产麻无矿码直接观看| 狼色精品人妻在线视频| 日韩乱码人妻无码系列中文字幕 | 久在线视视频在线观看| 欧美成人一区二区三区在线观看 | 一个添下面两个吃奶把腿扒开| 另类内射国产在线| 国内外精品激情刺激在线| 一級特黃色毛片免費看| 国产无套精品一区二区| 国产欧美综合一区二区三区| 国产精品综合一区二区三区| 天天爽夜夜爽人人爽88| 加勒比久久综合网天天| 黑人巨大跨种族video| 免费国产在线一区二区| 亚洲色成人一区二区三区| 丰满的少妇xxxxx青青青| 男人撕开奶罩揉吮奶头视频| 国产美女被遭高潮免费网站| 欧美日韩国产精品| 日日拍夜夜嗷嗷叫国产| 久久综合精品国产一区二区三区无码| 羞羞影院午夜男女爽爽免费| 忍不住的亲子中文字幕|