在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Human Aldehyde oxidase (AOX1), partial

In Stock
  • 中文名稱:
    Recombinant Human Aldehyde oxidase(AOX1),partial
  • 貨號:
    CSB-EP001858HU
  • 規(guī)格:
    ¥1344
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    AOX1; AOAldehyde oxidase; EC 1.2.3.1; Aldehyde oxidase 1; Azaheterocycle hydroxylase; EC 1.17.3.-
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    24.6kDa
  • 表達區(qū)域:
    236-421aa
  • 氨基酸序列
    FGSERMMWFSPVTLKELLEFKFKYPQAPVIMGNTSVGPEVKFKGVFHPVIISPDRIEELSVVNHAYNGLTLGAGLSLAQVKDILADVVQKLPEEKTQMYHALLKHLGTLAGSQIRNMASLGGHIISRHPDSDLNPILAVGNCTLNLLSKEGKRQIPLNEQFLSKCPNADLKPQEILVSVNIPYSRK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

 常見問題解答
Q:

Can you please provide the following:
1)For catalog: CSB-EP001858HU (all expression systems):
a.Has the tag been removed successfully for any expression systems? If yes, which ones?; b.Do you have any metabolism studies related data? Or knowledge on using this protein in metabolism studies? What co-factors are involved? What positive control can I use? (I don’t expect you to know this Vivian but thought I’d ask just in case you may.)
2)I am also looking for the following isoforms you may have, 2,3,4,etc. :
a.Recombinant Human Aldehyde oxidase 2 (full-length); b.Recombinant Human Aldehyde oxidase 3 (full-length); c.Recombinant Human Aldehyde oxidase 4 (full-length)
If you have these proteins, can you please also provide the same information requested above for AO a.Sequence; b.Tag type; c.Tag position; d.Sizes and formats; e.Has the tag been removed successfully for any expression systems? If yes, which ones?; f.Do you have any metabolism studies related data?...

A:
Thanks for your inquiry.
Recombinant human Aldehyde oxidase(AOX1)
CSB-YP001858HU >> Yeast
CSB-EP001858HU >> E.coli
CSB-BP001858HU >> Baculovirus
CSB-MP001858HU >> Mammalian cell
Sorry, we have never removed the tag of this protein before, We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%, but we should explaing to you in advance:
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
Please refer to the following link, it have the information of the metabolism studies: http://dmd.aspetjournals.org/content/38/8/1322.long
About the Aldehyde oxidase, the human species only express AOX1, so we don't have 2,3,4.

靶點詳情

  • 功能:
    Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
  • 基因功能參考文獻:
    1. AOX1 SNPs exist in which the base-pair exchange of the SNP leads to loss of enzyme activity. PMID: 26842593
    2. ten novel single nucleotide polymorphisms resulting in amino acid exchanges in proximity to the FAD site of hAOX1, were characterized. PMID: 28750088
    3. Data suggest that substrate specificity of AOX1 includes GDC-0834, an inhibitor of Bruton's tyrosine kinase with potential non-steroidal anti-inflammatory agent activity; in liver cytosol, hydrolysis of GDC-0834 is primarily due to AOX1. PMID: 25845827
    4. Methotrexate is poorly metabolized by human AOX1, in contrast to rabbit. PMID: 26032640
    5. These results supported the possibility that XO/XD and AO can contribute to nitric oxide generation. PMID: 25537183
    6. Several donors have a normal AOX1 protein level. PMID: 23857892
    7. High AOX1 methylation was associated with biochemical recurrence in prostate cancer. PMID: 23918943
    8. The physiological role of AOX1 remains unclear, but it is known that thiopurines belong to its substrates; AOX1 contributes to azathioprine catabolism. PMID: 22495427
    9. hydralazine, at a concentration of 25 to 50 muM, can be used in human hepatocyte incubations to estimate the contribution of aldehyde oxidase to the hepatic clearance of drugs and other compounds. PMID: 22522748
    10. Human population is characterized by the presence of functionally inactive hAOX1 allelic variants as well as variants encoding enzymes with different catalytic activities. PMID: 22279051
    11. characterization of AO activity in cryopreserved human hepatocytes: enzyme stability; substrate specificity; kinetics; inhibition by hydralazine PMID: 22031625
    12. Expressed AOX1 in Escherichia coli and purified kinetically active protein. PMID: 19741035

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Xanthine dehydrogenase family
  • 組織特異性:
    Abundant in liver, expressed in adipose tissue and at lower levels in lung, skeletal muscle, pancreas. In contrast to mice, no significant gender difference in AOX1 expression level (at protein level).
  • 數(shù)據(jù)庫鏈接:

    HGNC: 553

    OMIM: 602841

    KEGG: hsa:316

    STRING: 9606.ENSP00000363832

    UniGene: Hs.406238



主站蜘蛛池模板: 国产无遮挡乱子伦免费精品| 苍井空亚洲精品aa片在线播放| 中文乱码免费一区二区| 邻居少妇张开腿让我爽了一夜| 久久精品伊人一区二区三区| 日日噜噜夜夜狠狠va视频v| 女人色极品影院| 欧美婷婷丁香五月社区 | 久久精品国产99国产精2021| 任我爽精品视频在线播放| 西西人体444www大胆无码视频| 99日本精品永久免费久久| 少妇精品视频无码专区| 性久久久久久久| 欧美96在线 | 欧| 2021最新国产在线人成| 欧美日韩精品一区二区在线观看| 4hu四虎永久免费地址ww416| 国产精品色情国产三级在| 久久只有这里有精品4| 性色av无码不卡中文字幕| 亚洲第一综合天堂另类专| 亚洲欧洲国产码专区在线观看| 怡红院成永久免费人视频新的| 成人做爰69片免费看网站| 亚洲妇女水蜜桃av网网站| 狼友网精品视频在线观看 | 久久精品国产导航| 狂野av人人澡人人添| 中文字幕精品亚洲无线码vr| 国产99在线 | 欧美| 女人和拘做爰正片视频| 国产毛片一区二区精品| 婷婷伊人五月尤物| а天堂8中文最新版在线官网| 亚洲伊人久久大香线蕉| 狠狠综合久久久久尤物| 国精产品48x国精产品| 欧美日韩亚洲国产精品| 亚洲三区在线观看内射后入| 国产成人无码av大片大片在线观看 |