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Recombinant Escherichia coli Uncharacterized protein YjeA (poxA)

  • 中文名稱:
    大腸桿菌epmA重組蛋白
  • 貨號(hào):
    CSB-YP364196ENV
  • 規(guī)格:
  • 來(lái)源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌epmA重組蛋白
  • 貨號(hào):
    CSB-EP364196ENV
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌epmA重組蛋白
  • 貨號(hào):
    CSB-EP364196ENV-B
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌epmA重組蛋白
  • 貨號(hào):
    CSB-BP364196ENV
  • 規(guī)格:
  • 來(lái)源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌epmA重組蛋白
  • 貨號(hào):
    CSB-MP364196ENV
  • 規(guī)格:
  • 來(lái)源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    epmA
  • Uniprot No.:
  • 別名:
    epmA; genX; poxA; yjeA; b4155; JW4116Elongation factor P--(R)-beta-lysine ligase; EF-P--(R)-beta-lysine ligase; EC 6.3.1.-; EF-P post-translational modification enzyme A; EF-P-lysine lysyltransferase; GX
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度:
    full length protein
  • 表達(dá)區(qū)域:
    1-325
  • 氨基酸序列
    MSETASWQPS ASIPNLLKRA AIMAEIRRFF ADRGVLEVET PCMSQATVTD IHLVPFETRF VGPGHSQGMN LWLMTSPEYH MKRLLVAGCG PVFQLCRSFR NEEMGRYHNP EFTMLEWYRP HYDMYRLMNE VDDLLQQVLD CPAAESLSYQ QAFLRYLEID PLSADKTQLR EVAAKLDLSN VADTEEDRDT LLQLLFTFGV EPNIGKEKPT FVYHFPASQA SLAQISTEDH RVAERFEVYY KGIELANGFH ELTDAREQQQ RFEQDNRKRA ARGLPQHPID QNLIEALKVG MPDCSGVALG VDRLVMLALG AETLAEVIAF SVDRA
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF-P 'Lys-34'. The substrate (R)-beta-lysine is 100-fold more efficient than either (S)-beta-lysine or L-alpha-lysine. Cannot ligate lysine to any tRNA.
  • 基因功能參考文獻(xiàn):
    1. Misacylation of yeast amber suppressor tRNA(Tyr) is via E. coli lysyl-tRNA synthetase. PMID: 16672269
  • 蛋白家族:
    Class-II aminoacyl-tRNA synthetase family, EpmA subfamily
  • 數(shù)據(jù)庫(kù)鏈接:


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