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  4. Recombinant Escherichia coli Ubiquinol oxidase subunit 1 (cyoB), partial

Recombinant Escherichia coli Ubiquinol oxidase subunit 1 (cyoB), partial

  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 1(cyoB),partial
  • 貨號：
    CSB-YP359624ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 1(cyoB),partial
  • 貨號：
    CSB-EP359624ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 1(cyoB),partial
  • 貨號：
    CSB-EP359624ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 1(cyoB),partial
  • 貨號：
    CSB-BP359624ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 1(cyoB),partial
  • 貨號：
    CSB-MP359624ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cyoB
  • Uniprot No.：
  • 別名：
    cyoB; b0431; JW0421; Cytochrome bo(3) ubiquinol oxidase subunit 1; EC 7.1.1.3; Cytochrome b562-o complex subunit I; Cytochrome o ubiquinol oxidase subunit 1; Cytochrome o subunit 1; Oxidase bo(3) subunit 1; Ubiquinol oxidase chain A; Ubiquinol oxidase polypeptide I; Ubiquinol oxidase subunit 1
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron. Protons are probably pumped via D- and K- channels found in this subunit.
  • 基因功能參考文獻：
    1. selectively 15N labeled cyt bo3 samples have led to the unambiguous assignment of Nepsilon of R71 as the nitrogen that is strongly coupled to the QH site SQ in the wild-type enzyme and gives rise to the observed 14N ESEEM signals. PMID: 22497216
    2. Study determined the enzyme activity of cytochrome bo(3) (cbo(3))and the UQ-10 concentration in the membrane by cyclic voltammetry. PMID: 20096262
    3. Data show that the cytochrome bo3 tightly bound ubiquinone-8 at the Q(H) site is not displaced by ubiquinol-1 even during enzyme turnover. PMID: 20416270
    4. cytochrome bo requires Tyr288 for dioxygen reduction PMID: 15465820
    5. FTIR analysis of redox-induced protein structural changes in cytochrome bo PMID: 16040612
    6. analysis of heme-heme electron tunneling in the bo(3) enzyme, a bacterial relative to the mitochondrial cytochrome c oxidase PMID: 18087041

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  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Heme-copper respiratory oxidase family
  • 數據庫鏈接：


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