在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Threonine--tRNA ligase (thrS)

Recombinant Escherichia coli Threonine--tRNA ligase (thrS)

  • 中文名稱：
    大腸桿菌thrS重組蛋白
  • 貨號：
    CSB-YP364189ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌thrS重組蛋白
  • 貨號：
    CSB-EP364189ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌thrS重組蛋白
  • 貨號：
    CSB-EP364189ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌thrS重組蛋白
  • 貨號：
    CSB-BP364189ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌thrS重組蛋白
  • 貨號：
    CSB-MP364189ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    thrS
  • Uniprot No.：
  • 別名：
    thrS; b1719; JW1709; Threonine--tRNA ligase; EC 6.1.1.3; Threonyl-tRNA synthetase; ThrRS
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-642
  • 氨基酸序列
    MPVITLPDGS QRHYDHAVSP MDVALDIGPG LAKACIAGRV NGELVDACDL IENDAQLSII TAKDEEGLEI IRHSCAHLLG HAIKQLWPHT KMAIGPVIDN GFYYDVDLDR TLTQEDVEAL EKRMHELAEK NYDVIKKKVS WHEARETFAN RGESYKVSIL DENIAHDDKP GLYFHEEYVD MCRGPHVPNM RFCHHFKLMK TAGAYWRGDS NNKMLQRIYG TAWADKKALN AYLQRLEEAA KRDHRKIGKQ LDLYHMQEEA PGMVFWHNDG WTIFRELEVF VRSKLKEYQY QEVKGPFMMD RVLWEKTGHW DNYKDAMFTT SSENREYCIK PMNCPGHVQI FNQGLKSYRD LPLRMAEFGS CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE EQIRDEVNGC IRLVYDMYST FGFEKIVVKL STRPEKRIGS DEMWDRAEAD LAVALEENNI PFEYQLGEGA FYGPKIEFTL YDCLDRAWQC GTVQLDFSLP SRLSASYVGE DNERKVPVMI HRAILGSMER FIGILTEEFA GFFPTWLAPV QVVIMNITDS QSEYVNELTQ KLSNAGIRVK ADLRNEKIGF KIREHTLRRV PYMLVCGDKE VESGKVAVRT RRGKDLGSMD VNEVIEKLQQ EIRSRSLKQL EE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). The rate-limiting step is amino acid activation in the presence of tRNA. The 2'-OH of the acceptor base (adenine 76, A76) of tRNA(Thr) and His-309 collaborate to transfer L-Thr to the tRNA; substitution of 2'-OH of A76 with hydrogen or fluorine decreases transfer efficiency 760 and 100-fold respectively. The zinc ion in the active site discriminates against charging of the isosteric amino acid valine. Also activates L-serine, but does not detectably transfer it to tRNA(Thr). Edits incorrectly charged L-seryl-tRNA(Thr) via its editing domain, in a post-transfer reaction probably via water-mediated hydrolysis.; ThrS is also a translational repressor protein, it controls binds its own mRNA in the operator region upstream of the start codon. The mRNA region upstream of the start codon has a tRNA-like secondary structure; mRNA and tRNA compete for binding to ThrRS. ThrRS represses translation by preventing the ribosome from to mRNA, and tRNA(Thr) acts as an antirepressor allowing fine level control of enzyme synthesis. X-ray structures prove that operator mRNA and tRNA bind to overlapping sites in the protein.
  • 基因功能參考文獻：
    1. the relative contributions of pre- and post-transfer editing in ThrRS are subject to modulation by the rate of aminoacyl transfer PMID: 20504770
    2. Mutations in any of the three amino acids forming the zinc-binding site inactivate the enzyme and have a dominant negative effect on growth. PMID: 17644600
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family
  • 數據庫鏈接：


主站蜘蛛池模板： 国产成人精品无码一区二区三区| 狠狠色综合网丁香五月| 亚洲爆乳无码一区二区三区| 午夜性色福利在线观看视频| 在线天堂资源www在线中文| 国产自在自线午夜精品| 国精产品一品二品国精在线观看| 国产亚洲精品久久久网站好莱| 国产日韩欧美亚洲精品中字| 日韩精品一卡2卡3卡4卡新区| 国产18禁黄网站免费观看| 熟妇的味道hd中文字幕| 久久婷婷色香五月综合缴缴情| 99精品国产在热久久无毒| av鲁丝一区鲁丝二区鲁丝三区| 国产最新进精品视频| 性色欲情网站| 无码av无码天堂资源网影音先锋| 国产卡一卡二卡三| 奇米影视777四色狠狠| 日产精品卡1卡2卡三卡在线| 亚洲人亚洲人成电影网站色| 亚洲第一aaaaa片| 国产做爰xxxⅹ高潮视频12p| 婷婷色婷婷开心五月四房播播久久| 成人无码精品一区二区三区亚洲区| 成 人影片 免费观看| 国产精品高潮呻吟av久久小说| 大胆日本熟妇xxxx| 国产欧美一区二区三区在线看| 狠狠综合亚洲综合亚洲色| 久久www成人片免费看| 无码国产成人午夜电影在线观看| 无码中文字幕日韩专区| 国产毛片一区二区精品| 无码精品国产d在线观看| 18禁无遮挡免费视频网站| 老熟妇乱子交视频一区| 欧美日韩亚洲国产在线制服| 成人免费一区二区三区| 五月丁香综合缴情六月小说|