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  4. Recombinant Escherichia coli Ribonuclease E (rne), partial

Recombinant Escherichia coli Ribonuclease E (rne), partial

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  • 中文名稱：
    大腸桿菌rne重組蛋白
  • 貨號：
    CSB-EP324207ENV
  • 規格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    rne
  • Uniprot No.：
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    16.1 kDa
  • 表達區域：
    35-125aa
  • 氨基酸序列
    EQKKANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFISLAGS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. Can also process other RNA species, such as RNAI, a molecule that controls the replication of ColE1 plasmid, and the cell division inhibitor DicF-RNA. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic process. Required to initiate rRNA degradation during both starvation and quality control; acts after RNase PH (rph) exonucleolytically digests the 3'-end of the 16S rRNA. Degradation of 16S rRNA leads to 23S rRNA degradation. Processes the 3 tRNA(Pro) precursors immediately after the 3'-CCA to generate the mature ends.; Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates. 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated.
  • 基因功能參考文獻：
    1. This study suggests that, rather than its role in the processing of any one particular substrate, its distributed functions on all the different substrates (mRNA, rRNA, and tRNA) are responsible for the essentiality of RNase E in E. coli. PMID: 28167522
    2. Small RNAs processing and Hfq binding determinants for RNase E: RNase E cleaves any RNA fused to the 3' end. PMID: 26531825
    3. bacteria producing RNase E, have nutritional requirements different from those of cells supplied with only the N-terminal catalytic region of RNase E and mitigation of RNase E deficiency by overexpression of RNase G, is also affected by carbon source. PMID: 23275245
    4. Electrostatic potentials of were found to be conserved, highly positive, and spread over a large surface area encompassing four putative membrane-binding regions identified in the "large" domain. PMID: 22509045
    5. Strains harbouring rne alleles that express variants of RNase E in which 5' sensing or direct entry are inactivated, are viable. PMID: 22074454
    6. Increased abundance of R-loops in the rho and nusG mutants contributes to rescue the lethality associated with loss of the two RNase E cleavage pathways. PMID: 22026368
    7. demonstrated that CsdA, whose expression is induced by cold shock, interacts physically and functionally with RNase E PMID: 15554978
    8. The RNase E tetramer has two nonequivalent subunit interfaces, one of which is mediated by a single, tetrathiol-zinc complex; one or both interfaces organize the active site, which is distinct from the primary site of RNA binding. PMID: 15779893
    9. Observed role of RNase E in sodB mRNA turnover. PMID: 15781494
    10. crystal structures of the catalytic domain of RNase E as trapped allosteric intermediates with RNA substrates PMID: 16237448
    11. RNase E is required for induction of the glutamate-dependent acid resistance system in a RpoS-independent manner PMID: 17213667
    12. RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton PMID: 17242352
    13. This paper demonstrated that RNase E, a key endonuclease responsible for mRNA degradation in E. coli, regulates cspE transcript stability, possibly through the assembly of a degradosome. PMID: 18177308
    14. RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome PMID: 18337249
    15. RraA and RraB have a conserved property to directly act on RNAse E-related enzymes and inhibit their ribonucleolytic activity. PMID: 18510556
    16. Study shows that the arginine-rich RNA binding domain (ARRBD) of RNase E is important for the initial endoribonucleolytic cleavage of RNAI but dispensable for the endoribonucleolytic cleavages of the Rep mRNA. PMID: 19426759

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  • 亞細胞定位：
    Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    RNase E/G family, RNase E subfamily
  • 數據庫鏈接：


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