在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP)

Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP)

Unavailable
  • 中文名稱：
    Recombinant Escherichia coli Periplasmic serine endoprotease DegP(degP)
  • 貨號：
    CSB-EP314631ENV
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    degP
  • Uniprot No.：
  • 別名：
    degP; htrA; ptd; b0161; JW0157; Periplasmic serine endoprotease DegP; EC 3.4.21.107; Heat shock protein DegP; Protease Do
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    62.8kDa
  • 表達區(qū)域：
    27-474aa
  • 氨基酸序列
    AETSSATTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGNGGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRSDIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNAENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSSIFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKVLDSKPSVLALNIQRGDSTIYLLMQ
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The production of this recombinant E.coli degP protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the degP protein. N-terminal 6xHis-SUMO tag was used in the process. The purity is 90% determined by SDS-PAGE.

    degP is a gene providing instructions for making a protein called periplasmic serine endoprotease DegP (also known as heat shock protein DegP or protease Do). DegP protein belongs to the peptidase S1C family. DegP is a heat shock protein induced in response to pac overexpression, suggesting that the protein could possibly suppress the physiological toxicity caused by pac overexpression. Increasing evidence indicates that the production of soluble recombinant penicillin acylase in Escherichia coli can be enhanced via coexpression of a periplasmic protease/chaperone, DegP.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. Degrades transiently denatured and unfolded or misfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, Ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).
  • 基因功能參考文獻：
    1. Our results suggest that YjfN is a novel "suicide activator" for DegP that enhances DegP proteolysis under misfolded protein stress. PMID: 28947671
    2. Investigated the influence of amino acid substitutions located within the LD loop on the kinetics of a model substrate cleavage as well as on the dynamics of the oligomeric structure of HtrA. We found that the mutations that were expected to disturb the loop's structure and/or interactions with the remaining regulatory loops severely diminished the proteolytic activity of HtrA. PMID: 27469236
    3. In the present review, we summarize recent advances in the characterization of these key factors, with a special emphasis on the multifunctional protein DegP. PMID: 25849907
    4. The lack of the disulfide within LA affected the stability and the overall structure of the HtrA molecule. PMID: 25710793
    5. Sensitive activation and cage assembly needs covalent linkage of distinct substrate sequences that affect degradation (degrons); 1 degron binds the DegP active site, a 2nd degron binds a separate tethering site in PDZ1 of a substrate-bound DegP dodecamer. PMID: 21458668
    6. Formation of contacts between the apical parts (residues 231-234) of the L2 loops within the HtrA trimer, in particular the residue D232, was shown to play a crucial role in the activation process of HtrA. PMID: 20515644
    7. Effector-peptide binding to the PDZ domain of DegP induces oligomer conversion from resting hexameric DegP6 into proteolytically active 12-mers and 24-mers. PMID: 20581825
    8. The combined introduction of loop L2 and PDZ domain 1 of DegP into DegS converted DegS even further into a DegP-like protease. PMID: 20184896
    9. the osmoregulation of the expression of htrA gene; role of the nucleoid associated proteins H-NS and Hha in the repression of htrA expression at low osmolarity PMID: 16143461
    10. the N-terminal alpha-helix is an important functional domain for inhibition of the Cpx pathway and that CpxP is subject to DegP-dependent proteolysis PMID: 16166523
    11. CpxP functions as a periplasmic adaptor protein that is required for the effective proteolysis of a subset of misfolded substrates by the DegP protease PMID: 16303867
    12. Results show that the functional unit for the protease and chaperone activities of DegP is a trimer and that neither a cavity of specific dimensions nor the presence of an enclosed cavity appears to be essential for its protease and chaperone activities. PMID: 17122339
    13. HtrA may protect the bacterial cells from deleterious effects of heat shock not only by degrading the damaged proteins but by combination of the proteolytic and chaperoning activities PMID: 17485069
    14. This article summarizes recent studies regarding the HtrA family of proteins, their structure, regulation and function. It also presents practical applications of this knowledge and perspective of its use in the future. PMID: 17718385
    15. HtrA can in fact act as a protease at low temperatures. PMID: 19047732
    16. Review show that DegP adopts various forms, depending on the surrounding environment and the availability of a substrate. PMID: 19465652

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Peptidase S1C family
  • 數(shù)據(jù)庫鏈接：


主站蜘蛛池模板： 午夜理理伦电影a片无码| 国产午夜理论不卡在线观看| 99国产精品欧美一区二区三区 | 俺去俺来也在线www色官网| 国产亚洲色婷婷久久99精品| 日本免费一区二区三区日本| 少妇和邻居做不戴套视频| 久久97超碰色中文字幕蜜芽| 国产二级一片内射视频插放| 色爱区综合五月激情| 久久不见久久见免费视频6无删减 丰满日韩放荡少妇无码视频 | 国产v在线最新观看视频| 精品熟女少妇av免费久久| 日日狠狠久久8888偷偷色| 国内精品视频自在一区| 婷婷色综合视频在线观看| 日韩中文亚洲欧美视频二| 久久精品国产丝袜人妻| 国产亚洲情侣一区二区无| 欧美日产国产新一区| 人妻av无码中文专区久久| 熟女内射v888av| 精品噜噜噜噜久久久久久久久| 久久国产偷任你爽任你| 日本熟妇乱人伦a片免费高清 | 99精品人妻无码专区在线视频区| 成人亚洲欧美一区二区三区| 18禁止午夜福利体验区| 日韩国产人妻一区二区三区| 国产精品美女久久久久久久| 99久久国产自偷自偷免费一区| 少妇高潮无套内谢麻豆传| 免费国产裸体美女视频全黄| 无码中文字幕色专区| 日韩国产精品无码一区二区三区| 国产日韩综合av在线观看一区| 欧美人与zoxxxx另类| 国产92成人精品视频免费| 午夜福利体验免费体验区| 国产超碰人人做人人爱| 亚洲人成网站色www|