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  4. Recombinant Escherichia coli HTH-type transcriptional regulator gadX (gadX)

Recombinant Escherichia coli HTH-type transcriptional regulator gadX (gadX)

  • 中文名稱：
    大腸桿菌gadX重組蛋白
  • 貨號：
    CSB-YP330745ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌gadX重組蛋白
  • 貨號：
    CSB-EP330745ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌gadX重組蛋白
  • 貨號：
    CSB-EP330745ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌gadX重組蛋白
  • 貨號：
    CSB-BP330745ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌gadX重組蛋白
  • 貨號：
    CSB-MP330745ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gadX
  • Uniprot No.：
  • 別名：
    gadX; yhiX; b3516; JW3484; HTH-type transcriptional regulator GadX
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-274
  • 氨基酸序列
    MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN FVFVSRDTLS LFLPMLKEEA LNLHAHKKVS SLLVHHCSRD IPVFQEVAQL SQNKNLRYAE MLRKRALIFA LLSVFLEDEH FIPLLLNVLQ PNMRTRVCTV INNNIAHEWT LARIASELLM SPSLLKKKLR EEETSYSQLL TECRMQRALQ LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ ERSAQRLSNR DSAASIVAQG NFYGTDRSAE GIRL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW.
  • 基因功能參考文獻：
    1. Through biological and biochemical analysis, authors have demonstrated the GadX can directly interact with btuB promoter and affect the expression of btuB. PMID: 21314918
    2. The authors found that the overexpression of the GadX and GadY regulators of the glutamate-dependent acid resistance system complemented the growth defect of YidC-depleted cells. PMID: 21216990
    3. gadX is transcribed in an operon with gadW, although this full-length mRNA does not accumulate. PMID: 21147125
    4. The results indicate that the GadX regulator increases multidrug resistance in E. coli by activating the MdtEF multidrug efflux pump PMID: 18297445
    5. GadX/GadW-dependent activation of the acid fitness island is controlled at the gadX-gadW intergenic region, in which the gadY gene is located and transcribed divergently from gadW. PMID: 18808381

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