在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Elongation factor P (efp)

  • 中文名稱:
    大腸桿菌efp重組蛋白
  • 貨號:
    CSB-EP358853ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    efp
  • Uniprot No.:
  • 別名:
    efp; b4147; JW4107Elongation factor P; EF-P
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    36.5kDa
  • 表達區域:
    2-188aa
  • 氨基酸序列
    ATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLRRLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGDNAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGAVVKVPLFVQIGEVIKVDTRSGEYVSRVK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis.
  • 基因功能參考文獻:
    1. The activity of EF-P is controlled by recognition elements in the tRNA D-arm. PMID: 27216360
    2. study of the spatial distribution and ribosome-binding dynamics of EF-P in live E. coli cells; EF-P binds to and unbinds from translating ribosomes during at least 25% of all elongation events; it may bind during every elongation cycle PMID: 28588135
    3. Binding of EF-P stabilizes the P-site tRNA, particularly via interactions between its modification and the CCA end, thereby enforcing an alternative conformation of the polyproline-containing nascent chain, which allows a favorable substrate geometry for peptide bond formation. PMID: 29100052
    4. In the absence of EF-P, the presence of Rho utilization (rut) sites led to an ~30-fold decrease in translation of polyproline-encoding mRNAs. PMID: 27624127
    5. These findings indicate that the ability of a given PPX motif to initiate an EF-P-alleviated stall is strongly influenced by its local context. PMID: 25144653
    6. Authors demonstrate that EF-P is dispensable in Escherichia coli and Pseudomonas aeruginosa under laboratory growth conditions. PMID: 23591475
    7. Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P. PMID: 24003132
    8. EF-P is an elongation factor that enhances translation of polyproline-containing proteins: In the absence of EF-P, ribosomes stall at polyproline stretches, whereas the presence of EF-P alleviates the translational stalling. PMID: 23239623
    9. EF-P and its eukaryotic homolog, eIF5A, are essential for the synthesis of a subset of proteins containing proline stretches in all cells. PMID: 23239624
    10. GenX-elongation factor P-lysyl adenylate analogue crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a=105.93, b=102.96, c=119.94 A, beta=99.4 degrees, and diffracted to 2.5 A resolution PMID: 20823541
    11. reveal that the translation elongation factor P (EF-P) lysylation by GenX is enhanced by YjeK (lysine 2,3-aminomutase paralog) PMID: 20729861

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Elongation factor P family
  • 數據庫鏈接:


主站蜘蛛池模板: 麻豆人妻无码性色av专区| 99久久精品国产波多野结衣 | 少妇伦子伦精品无码styles| 在线亚洲日产一区二区| 看国产一毛片在线看手机看| 国产无遮挡a片又黄又爽漫画| 熟妇高潮精品一区二区三区 | 国产在线观看码高清视频| 正在播放重口老熟女露脸| 97人人添人澡人人爽超碰| 久久996re热这里有精品| 日本中文字幕亚洲乱码| 国产午夜亚洲精品不卡在线观看| 妺妺窝人体色www在线| 伊人精品一本久久综合| 熟女丝袜潮喷内裤视频网站| 一本一久本久a久久精品综合| 精品亚洲国产成人av| 中文字幕亚洲乱码熟女在线| 四虎影视精品永久在线观看| 日产精品1区2区3区| 亚洲精品1卡2卡三卡23卡| 国产高颜值大学生情侣酒店| 亚洲精品乱码久久久久红杏| 秋秋影视午夜福利高清| 成人福利国产精品视频| 国产乱码一区二区三区| 巨大黑人极品videos精品| 天堂а√中文最新版地址在线| 色惰日本视频网站www| 少妇人妻偷人激情视频| 春色校园综合人妻av| 久久香综合精品久久伊人| 久精品视频在线观看免费| 99久久免费国产精精品| 国产高潮国产高潮久久久| 午夜精品电影你懂的| 亚洲日韩精品无码专区加勒比海| 一日本道a高清免费播放| 亚洲另类无码专区国内精品| 亚洲色欲色欲天天天www|