在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli DNA ligase (ligA)

  • 中文名稱:
    大腸桿菌ligA重組蛋白
  • 貨號:
    CSB-EP322745ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    ligA
  • Uniprot No.:
  • 別名:
    ligA; dnaL; lig; lop; pdeC; b2411; JW2403; DNA ligase; EC 6.5.1.2; Polydeoxyribonucleotide synthase [NAD(+)]
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    77.6kDa
  • 表達區域:
    1-671aa
  • 氨基酸序列
    MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
  • 基因功能參考文獻:
    1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
    2. Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
    3. temperature sensitive mutant characterized PMID: 15583169
    4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
    5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
    6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
    7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356

    顯示更多

    收起更多

  • 蛋白家族:
    NAD-dependent DNA ligase family, LigA subfamily
  • 數據庫鏈接:


主站蜘蛛池模板: 人妻无码一区二区不卡无码av| 久久精品国产免费播| 国产呻吟久久久久久久92| 亚洲一区二区三区香蕉| 亚洲热无码av一区二区东京热av| 国产1卡2卡3卡4卡免费| 中文字幕亚洲一区二区三区| 亚洲精品国产精品国自产| 日本熟妇色xxxxxhd| 狠狠色噜噜狠狠狠狠蜜桃| 激情按摩系列片aaaa| 久9re热视频这里只有精品| 在线观看片免费人成视频播放| 亚洲精品四区麻豆文化传媒| 无码人妻精品一二三区免费| 对白脏话肉麻粗话av| 天堂官网在线资源网| 夜夜天天噜狠狠爱2019| 国产成人无码a区精油按摩| 日本黄页网站免费观看| 日本高清在线天码一区播放| 日韩一区精品视频一区二区| 亚洲国产精品综合久久网络| 少妇精油按摩av无码中字| 午夜精品影视国产一区在线麻豆 | 寂寞人妻瑜伽被教练日| 欧美性猛交xxxx乱大交蜜桃| 在线视频免费观看爽爽爽| 大香线蕉伊人超碰| 亚洲图片小说激情综合| 开心久久婷婷综合中文字幕| 亚洲国产成人精品青青草原| 激情文学小说区另类小说同性 | 亚洲欧美一区二区三区| 中国做爰国产精品视频| 插b内射18免费视频| 香港三级韩国三级日本三级 | 成年片色大黄全免费软件到| 亚洲欧洲日产国码无码网站| 一区二区三区在线 | 欧洲| 国产亚洲精品a在线无码|