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ligA Antibody, Biotin conjugated

Rare Species
  • 中文名稱:
    ligA兔多克隆抗體, Biotin偶聯
  • 貨號:
    CSB-PA322745HD01ENV
  • 規格:
    ¥880
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Escherichia coli ligA Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    ligA
  • 別名:
    ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Escherichia coli
  • 免疫原:
    Recombinant Escherichia coli DNA ligase protein (1-671AA)
  • 免疫原種屬:
    Escherichia coli
  • 標記方式:
    Biotin
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    >95%, Protein G purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
  • 基因功能參考文獻:
    1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
    2. Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
    3. temperature sensitive mutant characterized PMID: 15583169
    4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
    5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
    6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
    7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356

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  • 蛋白家族:
    NAD-dependent DNA ligase family, LigA subfamily
  • 數據庫鏈接:


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