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  4. Recombinant Escherichia coli Chemotaxis protein CheW (cheW)

Recombinant Escherichia coli Chemotaxis protein CheW (cheW)

  • 中文名稱(chēng)：
    Recombinant Escherichia coli Chemotaxis protein CheW(cheW)
  • 貨號(hào)：
    CSB-YP364282ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Escherichia coli Chemotaxis protein CheW(cheW)
  • 貨號(hào)：
    CSB-EP364282ENV-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    Recombinant Escherichia coli Chemotaxis protein CheW(cheW)
  • 貨號(hào)：
    CSB-BP364282ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Escherichia coli Chemotaxis protein CheW(cheW)
  • 貨號(hào)：
    CSB-MP364282ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cheW
  • Uniprot No.：
  • 別名：
    cheW; b1887; JW1876Chemotaxis protein CheW
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-167
  • 氨基酸序列
    MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN TPAFIKGVTN LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI VVDGVSDVLS LTAEQIRPAP EFAVTLSTEY LTGLGALGDR MLILVNIEKL LNSEEMALLD SAASEVA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.
  • 基因功能參考文獻(xiàn)：
    1. Results suggest that ligand binding triggers changes in the receptor that alter its signalling contacts with CheW. PMID: 25060668
    2. Co-overexpression of Tsr, CheA, and CheW yields hexagonal Arrays. PMID: 24580139
    3. analysis of CheA and CheW protein architecture and their role in bacterial chemotaxis PMID: 22355139
    4. The exchange of CheA and CheW at receptor clusters shows a modest dependency on the receptor modification. PMID: 21978288
    5. Results identified CheW surfaces responsible for CheA-Tsr interaction. PMID: 21463637
    6. The 2[to]2[to]1 organization is the core structural and functional unit of chemotaxis signaling complexes and hexagonal arrays characteristic of signaling complexes may be built from this unit. PMID: 21606342
    7. The authors propose that the CheW-binding sites in receptor dimers overlap their trimer contact sites and that high levels of CheW saturate the receptor-binding sites, preventing trimer assembly. PMID: 20487303
    8. The synergistic effect of serine and CheW binding to Tsr is attributed to distinct influences on receptor structure PMID: 15766389
    9. chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW PMID: 17360429

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 數(shù)據(jù)庫(kù)鏈接：


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