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  4. Recombinant Escherichia coli Chaperone protein skp (skp)

Recombinant Escherichia coli Chaperone protein skp (skp)

  • 中文名稱：
    大腸桿菌skp重組蛋白
  • 貨號：
    CSB-YP365201ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌skp重組蛋白
  • 貨號：
    CSB-EP365201ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌skp重組蛋白
  • 貨號：
    CSB-EP365201ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌skp重組蛋白
  • 貨號：
    CSB-BP365201ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌skp重組蛋白
  • 貨號：
    CSB-MP365201ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    skp
  • Uniprot No.：
  • 別名：
    skp; hlpA; ompH; b0178; JW0173; Chaperone protein Skp; DNA-binding 17 kDa protein; Histone-like protein HLP-1; Seventeen kilodalton protein
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    21-161
  • 氨基酸序列
    ADKIAIVNMG SLFQQVAQKT GVSNTLENEF KGRASELQRM ETDLQAKMKK LQSMKAGSDR TKLEKDVMAQ RQTFAQKAQA FEQDRARRSN EERGKLVTRI QTAVKSVANS QDIDLVVDAN AVAYNSSDVK DITADVLKQV K
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Molecular chaperone that interacts specifically with outer membrane proteins, thus maintaining the solubility of early folding intermediates during passage through the periplasm. Required for the efficient release of OmpA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of OmpA into the outer membrane.
  • 基因功能參考文獻：
    1. CpxQ combats toxicity at the inner membrane by downregulating the synthesis of the periplasmic chaperone Skp. PMID: 27048800
    2. Bioinformatic analysis of amino acid conservation, structural analysis of LPS-binding proteins, and MD simulations confirm the absence of a specific lipopolysaccharide binding site on Skp, and reveal a highly conserved salt-bridge network PMID: 25809264
    3. The global lifetime of the OmpX-Skp and tOmpA-Skp chaperone-substrate complex is seven orders of magnitude longer, emerging from the short local lifetimes by avidity. PMID: 24077225
    4. Evidence presented suggests that DegP/Skp function to rescue OMPs that fall off the SurA pathway. PMID: 17908933
    5. Seventeen kDa protein (Skp) interactions of Skp-OmpA complexes with lipopolysaccharide change the conformation of outer mebrane protein A (OmpA) in the Skp complex for facilitated insertion and folding into membranes. PMID: 19382746

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  • 亞細胞定位：
    Periplasm.
  • 蛋白家族：
    Skp family
  • 數據庫鏈接：


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