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  4. Recombinant Escherichia coli CRISPR system Cascade subunit CasE (casE)

Recombinant Escherichia coli CRISPR system Cascade subunit CasE (casE)

  • 中文名稱：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasE(casE)
  • 貨號：
    CSB-YP669387ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasE(casE)
  • 貨號：
    CSB-EP669387ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasE(casE)
  • 貨號：
    CSB-EP669387ENV-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasE(casE)
  • 貨號：
    CSB-BP669387ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasE(casE)
  • 貨號：
    CSB-MP669387ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    casE
  • Uniprot No.：
  • 別名：
    casE; cas6e; ygcH; b2756; JW2726CRISPR system Cascade subunit CasE; EC 3.1.-.-; CasE endoRNase; crRNA endonuclease
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-199
  • 氨基酸序列
    YLSKVIIAR AWSRDLYQLH QGLWHLFPNR PDAARDFLFH VEKRNTPEGC HVLLQSAQMP VSTAVATVIK TKQVEFQLQV GVPLYFRLRA NPIKTILDNQ KRLDSKGNIK RCRVPLIKEA EQIAWLQRKL GNAARVEDVH PISERPQYFS GDGKSGKIQT VCFEGVLTIN DAPALIDLVQ QGIGPAKSMG CGLLSLAPL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; CasE is required to process the pre-crRNA into single repeat-spacer units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. This subunit alone will cleave pre-crRNA, as will CasCDE or CasCE; cleavage does not require divalent metals or ATP. CasCDE alone is also able to form R-loops. Partially inhibits the cleavage of Holliday junctions by YgbT (Cas1). Yields a 5'-hydroxy group and a 2',3'-cyclic phosphate terminus.; A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization.
  • 基因功能參考文獻：
    1. The results show that in the E. coli subtype I-E system, CRISPR interference and primed adaptation can proceed in the absence of Cas6e endonucleolytic activity or in fact without the Cas6e protein itself. PMID: 26013814
  • 蛋白家族：
    CRISPR-associated protein Cas6/Cse3/CasE family, Subtype I-E/Ecoli subfamily
  • 數(shù)據(jù)庫鏈接：


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