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  4. Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial

Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial

  • 中文名稱：
    Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1(EBNA1) ,partial
  • 貨號：
    CSB-YP627067EEZ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1(EBNA1) ,partial
  • 貨號：
    CSB-EP627067EEZ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1(EBNA1) ,partial
  • 貨號：
    CSB-BP627067EEZ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1(EBNA1) ,partial
  • 貨號：
    CSB-MP627067EEZ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    EBNA1
  • Uniprot No.：
  • 別名：
    EBNA1; BKRF1; Epstein-Barr nuclear antigen 1; EBNA-1; EBV nuclear antigen 1
  • 種屬：
    Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Can you recommend a EBV-/Parvovirus - Peptide/Protein for use in cell culture (T-cell proliferation assay)
Here are the information provided for your reference:
- Searching for EBV-Proteins/Peptides and B19-ProteinsPeptides for use in cell culture (T-cell proliferation assay)
- Name/specific Target: EBV: EA, VCA, EBNA1
- Origin: if possible Protein/Peptide with viral origin. But E.Coli is OK as well
- Conjugation/Tag: if possible not conjugated, but tag is OK as well
- Size: 20 ug

A:
Thanks for your inquiry. EBNA1: Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1(EBNA1) ,partial,
CSB-YP627067EEZ >> Yeast
CSB-EP627067EEZ >> E.coli .
CSB-BP627067EEZ >> Baculovirus
CSB-MP627067EEZ >> Mammalian cell
Express region:458-641aa；C terminal partial.
Tag information:
YP,N terminal 6xHis-tagged；EP,BP,MP: N terminal 10xHis-tagged + C terminal Myc-tagged
Sequence:

RRKKGGWYGKHRGEGGSSQKFENIAEGLRLLLARCHVERTTEDGNWVAGVFVYGGSKTSLYNLRRGIGLAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFIVFLQTHIFAEGLKDAIKDLVLPKPAPTCNIKVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE


靶點詳情

  • 功能：
    Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival.
  • 基因功能參考文獻：
    1. PARP1-dependent PARylation of EBNA1 and adjacently bound TRF2 induces structural changes at the dyad symmetry element that reduce EBNA1 DNA binding affinity and functional recruitment of origin recognition complex. PMID: 20219917
  • 亞細胞定位：
    Host nucleus.
  • 蛋白家族：
    Herpesviridae EBNA1 family
  • 數據庫鏈接：

    KEGG: vg:5176225



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