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  4. Recombinant Dynein heavy chain, cytoplasmic (dhc-1), partial

Recombinant Dynein heavy chain, cytoplasmic (dhc-1), partial

  • 中文名稱：
    秀麗隱桿線蟲dhc-1重組蛋白
  • 貨號：
    CSB-YP619288CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲dhc-1重組蛋白
  • 貨號：
    CSB-EP619288CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲dhc-1重組蛋白
  • 貨號：
    CSB-EP619288CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲dhc-1重組蛋白
  • 貨號：
    CSB-BP619288CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲dhc-1重組蛋白
  • 貨號：
    CSB-MP619288CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dhc-1
  • Uniprot No.：
  • 別名：
    dhc-1; T21E12.4Dynein heavy chain; cytoplasmic; Dynein heavy chain; cytosolic; DYHC
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. May play a role in nuclear migration in hypodermal precursor cells. May be involved in the transport of synaptic vesicle components towards the axon of the DA motor neuron. This function may involve the regulation of dynein by pct-1 and/or cdk-5. Involved in the formation of synapses in the dorsal region during synaptic remodeling of DD motor neurons. Required for anterograde trafficking of dense-core vesicles in the DB motor neuron dendrites. Required for the formation of dendritic branches of PVD sensory neurons. May also play a role in GABAergic synaptic vesicle localization in the ventral nerve cord. May play a role in the pairing of homologous chromosomes during meiosis.
  • 基因功能參考文獻：
    1. Data indicate a role for DHC-1, the dynein heavy chain, in maintaining neuronal cargo distribution. PMID: 28406181
    2. Dynein tethered to the cell cortex generates pulling forces that position the mitotic spindle. Two distinct cortical dynein populations together create a robust force-generating system in the polarized one-cell Caenorhabditis elegans embryo. PMID: 28739679
    3. we dissect the role of dynactin's microtubule binding activity, located in the p150 CAP-Gly domain and an adjacent basic patch, in the C. elegans zygote. Our results suggest that p150 GAP-Gly domain binding to microtubules promotes initiation of dynein-mediated organelle transport in the dividing one-cell embryo, and that this function of p150 is critical for generating cytoplasmic pulling forces for centrosome PMID: 28759579
    4. Mutations affecting dhc-1 and cyb-3 cooperate to increase fitness in the absence of MDF-1. PMID: 25486568
    5. Data present evidence that bicd-1 operates in a conserved pathway with dhc-1 and unc-116. PMID: 21205795
    6. Dhc-1, as well as the dynactin components dnc-1 (p150Glued) and dnc-2 (p50/dynamitin), are essential for LIS-1 targeting to the nuclear periphery. PMID: 15331665
    7. mutational analysis of cytoplasmic dynein heavy chain in Caenorhabditis elegans PMID: 15616192
    8. Data show that accumulation of dynein at poles was kinesin-1 independent and APC dependent, just like dynein driven spindle movements. PMID: 19357192

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Dynein heavy chain family
  • 數據庫鏈接：

    KEGG: cel:CELE_T21E12.4

    STRING: 6239.T21E12.4

    UniGene: Cel.19011



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