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  4. Recombinant Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 (mbk-2), partial

Recombinant Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 (mbk-2), partial

  • 中文名稱：
    秀麗隱桿線蟲mbk-2重組蛋白
  • 貨號：
    CSB-YP895946CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mbk-2重組蛋白
  • 貨號：
    CSB-EP895946CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mbk-2重組蛋白
  • 貨號：
    CSB-EP895946CXY-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mbk-2重組蛋白
  • 貨號：
    CSB-BP895946CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mbk-2重組蛋白
  • 貨號：
    CSB-MP895946CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mbk-2
  • Uniprot No.：
  • 別名：
    mbk-2; F49E11.1Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2; EC 2.7.12.1; Dual specificity Yak1-related kinase mbk-2; Minibrain Kinase 2
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for oocyte-to-zygote transition in which it phosphorylates oocyte proteins, including mei-1, oma-1, oma-2, mex-5, and mex-6, modifying their activity and/or stability following meiosis. Functions in both spindle positioning and in the posterior localization of cytoplasmic determinants, including pie-1, pos-1, and pgl-1, in early embryos. Involved in the asymmetric distribution of plk-1 at the 2-cell embryonic stage.
  • 基因功能參考文獻：
    1. Phosphorylation by MBK-2 facilitates the binding of OMA proteins to TAF-4 and simultaneously inactivates their function in repressing zif-1 translation during the specification of primordial germ cells. PMID: 20826530
    2. MBK-2 functions as a temporal regulator of protein stability, and that coordinate activation of maternal protein degradation is one of the mechanisms that drives the transition from symmetric egg to patterned embryo. PMID: 12967564
    3. mbk-2 is essential in the zygote to ensure the postmeiotic inactivation of MEI-1 possibly via direct phosphorylation regulation PMID: 14634695
    4. MBK-2 down-regulates the katanin-related protein MEI-1 to control spindle positioning and, through yet unknown factors, to control the localization of cytoplasmic determinants. PMID: 14697358
    5. MBK-2 phosphorylates OMA-1 in vitro, primarily at threonine239; phosphorylation at T239 is required both for OMA-1 function in the 1-cell embryo and its degradation after the first mitosis PMID: 16289132
    6. MBK-2 directly phosphorylates MEI-1 and OMA-1 in vitro and that this activity is essential for their degradation in oocytes. PMID: 16338136

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  • 亞細胞定位：
    Cytoplasm, cell cortex.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
  • 組織特異性：
    In L1 larvae, expressed widely in the nervous system, including head neurons and the ventral nerve cord. In adult animals, continues to be expressed in the nervous system and is also expressed in body wall muscle.
  • 數據庫鏈接：

    KEGG: cel:CELE_F49E11.1

    STRING: 6239.F49E11.1b

    UniGene: Cel.39641



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