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  4. Recombinant Drosophila melanogaster cAMP-dependent protein kinase catalytic subunit (Pka-C1)

Recombinant Drosophila melanogaster cAMP-dependent protein kinase catalytic subunit (Pka-C1)

  • 中文名稱：
    黑腹果蠅Pka-C1重組蛋白
  • 貨號：
    CSB-YP318954DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Pka-C1重組蛋白
  • 貨號：
    CSB-EP318954DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Pka-C1重組蛋白
  • 貨號：
    CSB-BP318954DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Pka-C1重組蛋白
  • 貨號：
    CSB-MP318954DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pka-C1
  • Uniprot No.：
  • 別名：
    Pka-C1; CdkA; DC0; CG4379; cAMP-dependent protein kinase catalytic subunit 1; PKA C; EC 2.7.11.11; Protein kinase DC0
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-353
  • 氨基酸序列
    GNNATTSNK KVDAAETVKE FLEQAKEEFE DKWRRNPTNT AALDDFERIK TLGTGSFGRV MIVQHKPTKD YYAMKILDKQ KVVKLKQVEH TLNEKRILQA IQFPFLVSLR YHFKDNSNLY MVLEYVPGGE MFSHLRKVGR FSEPHSRFYA AQIVLAFEYL HYLDLIYRDL KPENLLIDSQ GYLKVTDFGF AKRVKGRTWT LCGTPEYLAP EIILSKGYNK AVDWWALGVL VYEMAAGYPP FFADQPIQIY EKIVSGKVRF PSHFGSDLKD LLRNLLQVDL TKRYGNLKAG VNDIKNQKWF ASTDWIAIFQ KKIEAPFIPR CKGPGDTSNF DDYEEEALRI SSTEKCAKEF AEF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Serine/threonine-protein kinase involved in memory formation. Promotes long-term memory by phosphorylating meng and by regulating CrebB protein stability and activity. As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology when anchored to the membrane.
  • 基因功能參考文獻：
    1. Hedgehog signalling activation causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex. PMID: 25289679
    2. tightly controlled dose-dependent intra-neuronal PKA activity levels are critical in determining the dendritic arbor complexity, one of the possible ways being through the regulation of organelle distribution. PMID: 25017992
    3. Alpha- NAC is a substrate of PKA following Parathyroid Hormone signaling. PMID: 24550008
    4. that manipulations that increase cAMP/PKA signaling in adipokinetic hormone (AKH)-producing cells at the base of the neuroendocrine ring gland restore the dNf1 growth deficiency. PMID: 24278035
    5. Acute expression of protein kinase A inhibitory peptide in aged animals is sufficient to reverse age-related memory impairment in wild-type animals. PMID: 21084612
    6. Two-dimensional gel electrophoresis, (32)P labelling and immunodetection show that "in vitro" PKA phosphorylates the serine in the C-terminus of the NDUFS4 subunit in isolated bovine complex I. PMID: 20433953
    7. Its signaling underlies age-related memory impairment.(review) PMID: 18646611
    8. We found that decreased PKA activity in neurons rendered flies insensitive to the wake-promoting effects of octopamine. PMID: 18799671

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  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
  • 組織特異性：
    More abundant in adult head than adult body.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4379

    STRING: 7227.FBpp0079448

    UniGene: Dm.2136



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